Abstract
A complete protocol is presented for the first time for the micropropagation of Pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Murashige and Skoog (MS) medium supplemented with 7.5 μM benzylaminopurine (BAP) induced up to 6.8 shoots per node with an average shoot length of 0.67 cm in 12 d. Incorporation of 2.5 μM gibberellic acid (GA3) in the medium during the first subculture after establishment and initiation of shoot buds significantly improved the shoot elongation. Single use of GA3 during the first subculture eliminated the need for prolonged culturing on BAP medium. Further use of GA3 in the medium was not useful. Shoot culture was established for at least two subcultures without loss of vigor by repeatedly subculturing the original cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 16–18 shoots were obtained in 60 d. Shoots formed in vitro were rooted on full-strength MS medium supplemented with 1.0 μM indole butyric acid (IBA). Plantlets were successfully acclimated, established in soil, and transferred to the nursery.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen ON, Allen EK (1981) The Leguminosae. The University of Wisconsin Press
Azam MM, Waris A, Nahar NM (2005) Prospects and potential of fatty acid methyl esters of some non-traditional seed oils for use as biodiesel in India. Biomass Bioenergy 29: 293–302
Bouza L, Jacques M, Miginiac E (1994) In vitro propagation of Paeonia suffruticosa ‘Andr. C.V. Mme de Vatry’: Developmental effects of exogenous hormones during the multiplication phase. Sci Hort 57: 241–251
Brassard N, Brissette L, Lord D, Laliberte S (1996) Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch. Plant Cell Tiss Org Cult 44: 37–44
Burkill JH (1966) A dictionary of economic products of the Malay peninsula. Art Printing Works, Kuala Lumpur
Demeke T, Hughes HG (1990) Micropropagation of Phytolacca dodecandra through shoot-tip and nodal cultures. Plant Cell Rep 9: 390–392
Duke J (1983) Handbook of Energy Crops
Edwards DGW, Naithani SC (1999) Seed and Nursery Technology of Forest Trees. New Age International, New Delhi
Figueiredo SFL, Albarello N, Viana VRC (2001) Micropropagation of Rollinia mucosa (Jacq.) Baill. In Vitro Cell Dev Biol Plant 37: 471–475
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Gopi C, Vatsala TM, Ponmurugan P (2006) In vitro multiple shoot proliferation and plant regeneration of Vanilla planifolia Andr.—A commercial spicy orchid. J Plant Biotech 8 (1): 37–41
Heller J (1996) Physic nut. Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. International Plant Genetic Resources Institute (IPGRI), Rome
Jordan M, Oyanedel E (1992) Regeneration of Pouteria lucuma (Sapotaceae) plants in vitro. Plant Cell Tiss Org Cult 31: 249–252
Karmee SK, Chadha A (2005) Preparation of biodiesel from crude oil of Pongamia pinnata. Bioresour Technol 96 (13): 1425–1429
Lloyd GB, McCown BH (1980) Commercially feasible micropropagation of mountain laural Kalmia latifolia by use of shoot tip culture. Proc Inter Plant Prop Soc 30: 421–427
Minocha SC (1987) pH of the medium and the growth and metabolism of cells in culture. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry. Martinus Nijhoff Pub, pp 125–141
Murashige T (1974) Plant propagation through tissue culture. Ann Rev Plant Physiol 25: 135–166
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Muthu C, Ayyanar M, Raja N, Ignacimuthu S (2006) Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. J Ethnobiol Ethnomed 2: 43
Naik SK, Pattnaik S, Chand PK (2000) High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Sci Hort 85: 261–270
NAS Firewood Crops (1980) Shrub and tree species for energy production. National Academy of Sciences, Washington, DC
Pradhan C, Kar S, Pattnaik S, Chand PK (1998) Propagation of Dalbergia sissoo Roxb. through in vitro shoot proliferation from cotyledonary nodes. Plant Cell Rep 18: 122–126
Punitha R, Vasudevan K, Manoharan S (2006) Effect of Pongamia pinnata flowers on blood glucose and oxidative stress in alloxan induced diabetic rats. Ind J Pharmcol 38 (1): 62–63
Purohit SD, Dave A (1996) Micropropagation of Sterculia urens Roxb. an endangered tree species. Plant Cell Rep 15: 704–706
Purohit SD, Singhvi A (1998) Micropropagation of Achras sapota through enhanced axillary branching. Sci Hort 76: 219–229
Shrinivasa U (2001) A viable substitute for diesel in rural India. Curr Sci 80: 1483–1484
Taiz L, Zeiger E (1998) Plant Physiology. 2nd ed. Sinauer Associates, Sunderland, Mass., USA
Acknowledgement
The authors thank the Head, Center for Energy at Indian Institute of Technology Guwahati for providing the necessary facilities.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: Woong-Young Soh
Rights and permissions
About this article
Cite this article
Sugla, T., Purkayastha, J., Singh, S.K. et al. Micropropagation of Pongamia pinnata through enhanced axillary branching. In Vitro Cell.Dev.Biol.-Plant 43, 409–414 (2007). https://doi.org/10.1007/s11627-007-9086-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-007-9086-x