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Msx-1 gene expression and regulation in embryonic palatal tissue

  • Growth, Differentiation And Senescence
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Summary

The palatal cleft seen in Msx-1 knock-out mice suggests a role for this gene in normal palate development. The cleft is presumed secondary to tooth and jaw malformations, since in situ hybridization suggests that Msx-1 mRNA is not highly expressed in developing palatal tissue. In this study we demonstrate, by Northern blot analysis, the expression of Msx-1, but not Msx-2, in the developing palate and in primary cultures of murine embryonic palate mesenchymal cells. Furthermore, we propose a role for Msx-1 in retinoic acid-induced cleft palate, since retinoic acid inhibits Msx-1 mRNA expression in palate mesenchymal cells. We also demonstrate that transforming growth factor beta inhibits Msx-1 mRNA expression in palate mesenchymal cells, with retinoic acid and transforming growth factor beta acting synergistically when added simultaneously to these cells. These data suggest a mechanistic interaction between retinoic acid, transforming growth factor beta, and Msx-1 in the etiology of retinoic acid-induced cleft palate.

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  1. Department of Biological and Biophysical Sciences, School of Dentistry, University of Louisville, 501 South Preston Street, 40292, Louisville, Kentucky

    Paul Nugent & Robert M. Greene

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  1. Paul Nugent
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Nugent, P., Greene, R.M. Msx-1 gene expression and regulation in embryonic palatal tissue. In Vitro Cell.Dev.Biol.-Animal 34, 831–835 (1998). https://doi.org/10.1007/s11626-998-0038-5

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  • DOI: https://doi.org/10.1007/s11626-998-0038-5

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