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Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)

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Abstract

Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain of golden pompano Trachinotus ovatus was established and characterized in this study. TOGB cell line showed high virus susceptibility, especially grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). As one of the most devastating viruses in marine fish aquaculture, nervous necrosis virus (NNV) causes high mortality rates exceeding 95% in severe outbreaks. Then, TOGB cell line was a useful tool for propagating viruses and provides a potentially valuable resource for the study of viral pathogenesis, the development of antiviral strategies. The TOGB cell lines showed potential application in environmental monitoring. The extracellular products from Vibrio anguillarum and Vibrio alginolyticus demonstrated cytotoxic effects in TOGB cells. TOGB cells grew most rapidly at 28°C, with an optimal concentration of 10% fetal bovine serum in L-15 medium. TOGB cells were diploid (2N = 54). The transfection efficiencies of TOGB cells were 8.6% at the 15th passage and 64.8% at the 45th passage, indicating that the cells are suitable for foreign gene expression.

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Acknowledgments

This study was supported by the National Basic Research Program of China (973) (2012CB114402) and grants from the National Natural Science Foundation of China (31330082) and grants from Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences (LMB141005).

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Authors and Affiliations

  1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China

    Pengfei Li, Lingli Zhou, Songwei Ni, Yepin Yu, Shina Wei & Qiwei Qin

  2. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China

    Pengfei Li, Lingli Zhou, Songwei Ni & Yepin Yu

  3. College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China

    Jia Cai

  4. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China

    Jia Cai

  5. State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, College of Marine Science, Hainan University, Haikou, 570228, China

    Meng Xu

Authors
  1. Pengfei Li
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  2. Lingli Zhou
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  3. Songwei Ni
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  4. Meng Xu
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  5. Yepin Yu
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  6. Jia Cai
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  7. Shina Wei
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  8. Qiwei Qin
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Corresponding authors

Correspondence to Shina Wei or Qiwei Qin.

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Editor: Tetsuji Okamoto

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Li, P., Zhou, L., Ni, S. et al. Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus). In Vitro Cell.Dev.Biol.-Animal 52, 410–418 (2016). https://doi.org/10.1007/s11626-015-9988-6

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  • DOI: https://doi.org/10.1007/s11626-015-9988-6

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