Skip to main content
SpringerLink
Log in

Cytoprotective effects of 6′-O-galloylpaeoniflorin against ultraviolet B radiation-induced cell damage in human keratinocytes

  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

Abstract

The cytoprotective effects of 6′-O-galloylpaeoniflorin against injury and death of human HaCaT keratinocytes resulting from ultraviolet B radiation were investigated. 6′-O-galloylpaeoniflorin exhibited the capacity to scavenge intracellular reactive oxygen species (ROS) generated by ultraviolet B radiation. 6′-O-galloylpaeoniflorin also attenuated ultraviolet B-induced oxidative macromolecular damage to DNA, lipids, and proteins, decreasing the number of DNA strand breaks, the level of 8-isoprostane (a biomarker of lipid peroxidation), and the level of protein carbonylation. Moreover, 6′-O-galloylpaeoniflorin rescued HaCaT cells from ultraviolet induced cell death, by downregulating the mitochondrial apoptotic pathway. Taken together, these results indicate that 6′-O-galloylpaeoniflorin has the potential to be developed as a medical agent against ROS-mediated skin diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.

Similar content being viewed by others

References

  • Afaq F (2011) Natural agents: cellular and molecular mechanisms of photoprotection. Arch Biochem Biophys 508:144–151

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Alfadda AA, Sallam RM (2012) Reactive oxygen species in health and disease. J Biomed Biotechnol. doi:10.1155/2012/936486; 2012

    PubMed Central  PubMed  Google Scholar 

  • Beauchamp MC, Letendre É, Renier G (2002) Macrophage lipoprotein lipase expression is increased in patients with heterozygous familial hypercholesterolemia. J Lipid Res 43:215–222

    CAS  PubMed  Google Scholar 

  • Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47:936–942

    CAS  PubMed  Google Scholar 

  • Chang H, Sander CS, Müller CS, Elsner P, Thiele JJ (2002) Detection of poly (ADP-ribose) by immunocytochemistry: a sensitive new method for the early identification of UVB-and H2O2-induced apoptosis in keratinocytes. Biol Chem 383:703–708

    Article  CAS  PubMed  Google Scholar 

  • Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R (2003) Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta 329:23–38

    Article  CAS  PubMed  Google Scholar 

  • de Gruijl FR, van Kranen HJ, Mullenders LH (2001) UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer. J Photochem Photobiol B 63:19–27

    Article  PubMed  Google Scholar 

  • F’guyer S, Afaq F, Mukhtar H (2003) Photochemoprevention of skin cancer by botanical agents. Photodermatol Photoimmunol Photomed 19:56–72

    Article  PubMed  Google Scholar 

  • Grewe M, Gyufko K, Krutmann J (1995) Interleukin-10 production by cultured human keratinocytes: regulation by ultraviolet B and ultraviolet A1 radiation. J Invest Dermatol 104:3–6

    Article  CAS  PubMed  Google Scholar 

  • Hampton MB, Orrenius S (1998) Redox regulation of apoptotic cell death. Bio Factors 8:1–5

    CAS  Google Scholar 

  • Hyun YJ, Piao MJ, Zhang R, Choi YH, Chae S, Hyun JW (2012) Photo-protection by 3-bromo-4, 5-dihydroxybenzaldehyde against ultraviolet B-induced oxidative stress in human keratinocytes. Ecotoxicol Environ Saf 83:71–78

    Article  CAS  PubMed  Google Scholar 

  • Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T (2003) UV-induced skin damage. Toxicology 189:21–39

    Article  CAS  PubMed  Google Scholar 

  • Ikehata H, Ono T (2011) The mechanisms of UV mutagenesis. J Radiat Res 52:115–125

    Article  CAS  PubMed  Google Scholar 

  • Jeong SY, Seol DW (2008) The role of mitochondria in apoptosis. BMB Rep 41:11–22

    Article  CAS  PubMed  Google Scholar 

  • Kang SS, Shin KH, Chi HJ (1991) Galloylpaeoniflorin, a new acylated monoterpene glucoside from paeony root. Arch Pharmacal Res 14:52–54

    Article  CAS  Google Scholar 

  • Liu S, Guo C, Wu D, Ren Y, Sun MZ, Xu P (2012) Protein indicators for HaCaT cell damage induced by UVB irradiation. J Photochem Photobiol B 114:94–101

    Article  CAS  PubMed  Google Scholar 

  • Matsuda H, Ohta T, Kawaguchi A, Yoshikawa M (2001) Bioactive constituents of Chinese natural medicines. VI. Moutan cortex. (2): structures and radical scavenging effects of suffruticosides A, B, C, D, and E and galloyl-oxypaeoniflorin. Chem Pharm Bull 49:69–72

    Article  CAS  PubMed  Google Scholar 

  • Mohamad N, Gutiérrez A, Núñez M, Cocca C, Martín G, Cricco G, Medina V, Rivera E, Bergoc R (2005) Mitochondrial apoptotic pathways. Biocell 29:149–161

    CAS  PubMed  Google Scholar 

  • Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139:271–279

    Article  CAS  PubMed  Google Scholar 

  • Nishi J, Ogura R, Sugiyama M, Hidaka T, Kohno M (1991) Involvement of active oxygen in lipid peroxide radical reaction of epidermal homogenate following ultraviolet light exposure. J Invest Dermatol 97:115–119

    Article  CAS  PubMed  Google Scholar 

  • Okimoto Y, Watanabe A, Niki E, Yamashita T, Noguchi N (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett 474:137–140

    Article  CAS  Google Scholar 

  • Punnonen K, Puntala A, Ahotupa M (1991) Effects of ultraviolet A and B irradiation on lipid peroxidation and activity of the antioxidant enzymes in keratinocytes in culture. Photodermatol Photoimmunol Photomed 8:3–6

    CAS  PubMed  Google Scholar 

  • Rajagopalan R, Ranjan SK, Nair CKK (2003) Effect of vinblastine sulfate on γ-radiation-induced DNA single-strand breaks in murine tissues. Mutat Res Genet Toxicol Environ Mutag 536:15–25

    Article  CAS  Google Scholar 

  • Ravanat JL, Douki T, Cadet J (2001) Direct and indirect effects of UV radiation on DNA and its components. J Photochem Photobiol B 63:88–102

    Article  CAS  PubMed  Google Scholar 

  • Ray PD, Huang BW, Tsuji Y (2012) Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 24:981–990

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rosenkranz AR, Schmaldienst S, Stuhlmeier KM, Chen W, Knapp W, Zlabinger GJ (1992) A microplate assay for the detection of oxidative products using 2’,7’-dichlorofluorescin-diacetate. J Immunol Methods 156:39–45

    Article  CAS  PubMed  Google Scholar 

  • Sander CS, Chang H, Hamm F, Elsner P, Thiele JJ (2004) Role of oxidative stress and the antioxidant network in cutaneous carcinogenesis. Int J Dermatol 43:326–335

    Article  CAS  PubMed  Google Scholar 

  • Sander CS, Chang H, Salzmann S, Müller CS, Ekanayake-Mudiyanselage S, Elsner P, Thiele JJ (2002) Photoaging is associated with protein oxidation in human skin in vivo. J Invest Dermatol 118:618–625

    Article  CAS  PubMed  Google Scholar 

  • Savini I, D’Angelo I, Ranalli M, Melino G, Avigliano L (1999) Ascorbic acid maintenance in HaCaT prevents radical formation and apoptosis by UV-B. Free Radical Biol Med 26:1172–1180

    Article  CAS  Google Scholar 

  • Scandalios JG (2002) The rise of ROS. Trends Biochem Sci 27:483–486

    Article  CAS  PubMed  Google Scholar 

  • Schwarz A, Bhardwaj R, Aragane Y, Mahnke K, Riemann H, Metze D, Luger TA, Schwarz T (1995) Ultraviolet-B-induced apoptosis of keratinocytes: evidence for partial involvement of tumor necrosis factor-α in the formation of sunburn cells. J Invest Dermatol 104:922–927

    Article  CAS  PubMed  Google Scholar 

  • Singh NP (2000) Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res Fundam Mol Mech Mutag 455:111–127

    Article  CAS  Google Scholar 

  • Stahl W, Sies H (2002) Carotenoids and protection against solar UV radiation. Skin Pharmacol Physiol 15:291–296

    Article  CAS  Google Scholar 

  • Tsujimoto Y, Shimizu S (2000) Bcl-2 family: life-or-death switch. FEBS Lett 466:6–10

    Article  CAS  PubMed  Google Scholar 

  • Washida K, Itoh Y, Iwashita T, Nomoto K (2009) Androgen modulators from the roots of Paeonia lactiflora (Paeoniae Radix) grown and processed in nara prefecture. Japan Chem Pharm Bull 57:971–974

    Google Scholar 

  • Wyllie A, Morris R, Smith A, Dunlop D (1984) Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142:67–77

    Article  CAS  PubMed  Google Scholar 

  • Yao CW, Piao MJ, Kim KC, Zheng J, Cha JW, Hyun JW (2013) 6’-O-Galloylpaeoniflorin protects human keratinocytes against oxidative stress-induced cell damage. Biomol Ther 21:349–357

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This research was supported by the 2014 scientific promotion program funded by Jeju National University.

Author information

Authors and Affiliations

  1. School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju, 690-756, Korea

    Cheng Wen Yao, Mei Jing Piao, Ki Cheon Kim, Jian Zheng, Ji Won Cha, Chang Lim Hyun, Sun Jin Boo, Soo Young Na, Suk Ju Cho & Jin Won Hyun

Authors
  1. Cheng Wen Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mei Jing Piao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ki Cheon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ji Won Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chang Lim Hyun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sun Jin Boo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Soo Young Na
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Suk Ju Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jin Won Hyun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin Won Hyun.

Additional information

Editor: T. Okamoto

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yao, C.W., Piao, M.J., Kim, K.C. et al. Cytoprotective effects of 6′-O-galloylpaeoniflorin against ultraviolet B radiation-induced cell damage in human keratinocytes. In Vitro Cell.Dev.Biol.-Animal 50, 664–674 (2014). https://doi.org/10.1007/s11626-014-9747-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11626-014-9747-0

Keywords

Search

Navigation