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The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells

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Abstract

Platelet-rich plasma (PRP) is an emerging therapeutic application because PRP contains various growth factors that have beneficial effects on tissue regeneration and engineering. Mesenchymal stem cells and PRP derived from peripheral blood have been well studied. In this study, we investigated the effects of PRP derived from human umbilical cord blood (UCB-PRP) on proliferation, alkaline phosphatase (ALP) activity, and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and periodontal ligament stem cells (PDLSCs). Three types of dental stem cells were primarily isolated and characterized by flow cytometric analysis. Dental stem cells were exposed to various concentrations of UCB-PRP, which resulted in the proliferation of dental stem cells. Treatment with 2% UCB-PRP resulted in the highest level of proliferation. The ALP activity of DPSCs and PDLSCs increased following treatment with UCB-PRP in a dose-dependent manner up to a concentration of 2%. ALP activity decreased with higher concentration of UCB-PRP. The effects of UCB-PRP on calcium deposition were similar to those on proliferation and ALP activity. Treatment with 2% UCB-PRP resulted in the highest calcium depositions in DPSCs and PDLSCs; however, treatment with 1% UCB-PRP resulted in the highest calcium deposition in SHEDs. The concentrations of platelet-derived growth factor-AB and transforming growth factor-β1 in UCB-PRP were investigated and found to be comparable to the amounts in peripheral blood. Overall, UCB-PRP had beneficial effects on the proliferation and osteogenic differentiation of dental stem cells. Determination of the optimal concentration of UCB-PRP requires further investigation for clinical applications.

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Acknowledgement

This work was supported by Bioteeth Project (no. 20090084043) from the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology, Republic of Korea and by Regenomics (no. 20100002086), the nanobiotechnology development program from the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology, Republic of Korea.

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Authors and Affiliations

  1. Department of Periodontology, School of Dentistry, Seoul National University, Seoul, 110-749, South Korea

    Jung-Yeon Lee, Chong-Pyoung Chung & Soo-Boo Han

  2. Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, 28 Yeongon-dong, Chongro-gu, Seoul, 110-749, South Korea

    Hyun Nam & Gene Lee

  3. Department of Dental Science, School of Dentistry, Seoul National University, Seoul, 110-749, South Korea

    Yoon-Jeong Park

  4. Department of Pharmacy, College of Pharmacy, Ewha Womans University, Seoul, 120-750, South Korea

    Seung-Jin Lee

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  1. Jung-Yeon Lee
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  2. Hyun Nam
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  4. Seung-Jin Lee
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  5. Chong-Pyoung Chung
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  6. Soo-Boo Han
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  7. Gene Lee
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Correspondence to Gene Lee.

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Editor: J. Denry Sato

Jung-Yeon Lee and Hyun Nam equally contributed to this work.

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Lee, JY., Nam, H., Park, YJ. et al. The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells. In Vitro Cell.Dev.Biol.-Animal 47, 157–164 (2011). https://doi.org/10.1007/s11626-010-9364-5

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  • DOI: https://doi.org/10.1007/s11626-010-9364-5

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