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The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue

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Abstract

The effect of Bombyx mori nuclear polyhedrosis virus (BmNPV) on biochemical changes of TC-100 medium containing 10% fetal bovine serum (FBS) in embryonic primary cultures of silkworm was investigated. The primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with TC-100 medium representing multiplicity of infection (MOI) of 0.25, 0.5, and 1). Glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the medium of BmNPV-infected primary cultures. All biochemical compounds showed significant changes. Glucose decreased considerably by about 55 mg/ml, while different concentrations of the virus inoculums did not demonstrate significant differences among them. Total protein had only increased in 2 ml concentration and there were no changes in other concentrations. Uric acid as a by-product accumulated dramatically in all concentrations, while the amount of urea reduced in all treatments and this reduction was more evident in lower concentrations. Cholesterol consumption was high in cultures postinfection, while alkaline phosphatase (ALP) activity decreased in infected cells.

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Acknowledgments

This work was supported by the University of Guilan and has been financed with the projects of the Department of Sericulture. We would like to thank the Iran Sericulture Research Center for their technical assistance.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran

    Leila Matindoost & Jalal Jalali Sendi

  2. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Hoorieh Soleimanjahi

  3. Department of Sericulture, Faculty of Natural Resources, The University of Guilan, Somehe Sara, P.O. Box 1144 , Rasht, Iran

    Kayvan Etebari

  4. Department of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Fateme Rahbarizade

Authors
  1. Leila Matindoost
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  2. Jalal Jalali Sendi
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  3. Hoorieh Soleimanjahi
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  4. Kayvan Etebari
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  5. Fateme Rahbarizade
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Correspondence to Kayvan Etebari.

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Editor: J. Denry Sato

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Matindoost, L., Sendi, J.J., Soleimanjahi, H. et al. The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue. In Vitro Cell.Dev.Biol.-Animal 44, 121–127 (2008). https://doi.org/10.1007/s11626-008-9083-3

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  • DOI: https://doi.org/10.1007/s11626-008-9083-3

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