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SENP3 Promotes Mantle Cell Lymphoma Development through Regulating Wnt10a Expression

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Abstract

Objective

SUMO-specific protease 3 (SENP3), a member of the SUMO-specific protease family, reverses the SUMOylation of SUMO-2/3 conjugates. Dysregulation of SENP3 has been proven to be involved in the development of various tumors. However, its role in mantle cell lymphoma (MCL), a highly aggressive lymphoma, remains unclear. This study was aimed to elucidate the effect of SENP3 in MCL.

Methods

The expression of SENP3 in MCL cells and tissue samples was detected by RT-qPCR, Western blotting or immunohistochemistry. MCL cells with stable SENP3 knockdown were constructed using short hairpin RNAs. Cell proliferation was assessed by CCK-8 assay, and cell apoptosis was determined by flow cytometry. mRNA sequencing (mRNA-seq) was used to investigate the underlying mechanism of SENP3 knockdown on MCL development. A xenograft nude mouse model was established to evaluate the effect of SENP3 on MCL growth in vivo.

Results

SENP3 was upregulated in MCL patient samples and cells. Knockdown of SENP3 in MCL cells inhibited cell proliferation and promoted cell apoptosis. Meanwhile, the canonical Wnt signaling pathway and the expression of Wnt10a were suppressed after SENP3 knockdown. Furthermore, the growth of MCL cells in vivo was significantly inhibited after SENP3 knockdown in a xenograft nude mouse model.

Conclusion

SENP3 participants in the development of MCL and may serve as a therapeutic target for MCL.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China

    Yan-ni Ma, Yun-ding Zou, Zhi-long Liu, Gui-xian Wu, Yuan-ze Zhou, Cheng-xin Luo, Xiang-tao Huang, Ming-ling Xie & Shuang-nian Xu

  2. Department of Hematology, the Third Affiliated Hospital (Daping Hospital), Third Military Medical University (Army Medical University), Chongqing, 400042, China

    Yan-ni Ma & Xi Li

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  1. Yan-ni Ma
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Correspondence to Xi Li.

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The authors have declared no competing interests in this work.

Additional information

This study was supported by the Chongqing Natural Science Foundation (No. 2023NSCQ-MSX3161 and No. cstc2020jcyj-msxmX1058) and the National Natural Science Foundation of China (No. 81800172).

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Ma, Yn., Zou, Yd., Liu, Zl. et al. SENP3 Promotes Mantle Cell Lymphoma Development through Regulating Wnt10a Expression. CURR MED SCI 44, 134–143 (2024). https://doi.org/10.1007/s11596-024-2829-7

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  • DOI: https://doi.org/10.1007/s11596-024-2829-7

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