Summary
The effects of essential oil from Carpesium abrotanoides L. (CAEO) on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study. MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79 μg/mL. Moreover, many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258 staining and fluorescence microscopy. Flow cytometry was used to detect cell apoptosis and cell cycle, and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases. The decreased Bcl-2/Bax protein ratio and the activation of caspase-3, caspase-9 were also detected by Western blotting. All results suggested that CAEO is a potential agent to fight against liver cancer, and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.
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Wang XK, Peng Y, Tao HR, et al. Inhibition of adhesion and metastasis of HepG2 hepatocellular carcinoma cells in vitro by DNA aptamer against Sialyl Lewis X. J Huazhong Univ Sci Technolog Med Sci, 2017,37(3):343–347
Befeler AS, Di Bisceglie AM. Hepatocellular carcinoma: diagnosis and treatment. Gastroenterology, 2002,122(6):1609–1619
Gao Y, Chen Y, Ji X, et al. Controlled intracellular release of doxorubicin in multidrug-resistant cancer cells by tuning the shell-pore sizes of mesoporous silica nanoparticles. ACS Nano, 2011,5(12):9788–9798
Hu Y, Wang S, Wu X, et al. Chinese herbal medicinederived compounds for cancer therapy: a focus on hepatocellular carcinoma. J Ethnopharmacol, 2013,149(3):601–612
Nobili S, Lippi D, Witort E, et al. Natural compounds for cancer treatment and prevention. Pharmacol Res, 2009,59(6):365–378
Wang JF, He WJ, Zhang XX, et al. Dicarabrol, a new dimeric sesquiterpene from Carpesium abrotanoides L. Bioorg Med Chem Lett, 2015,25(19):4082–4084
Liu PA, Liu M, Pan WW, et al. Study on chemical constituents from Carpesium abrotanoides. J Chin Med Mater, 2014,37(4):2213–2215
Lee SB, Kang K, Lee HJ, et al. The chemopreventive effects of Carpesium abrotanoides are mediated by induction of phase II detoxification enzymes and apoptosis in human colorectal cancer cells. J Med Food, 2010,13(1):39–46
Shen BB, Tang XY, Chen L, et al. Analysis of volatile constituents from aerial part of Carpesium abrotanoides by SPME-GC/MS. Mod Chin Med, 2017,19(7):965–971
Lv D, Guo K, Xu C, et al. Essential oil from Siegesbeckia pubescens induces apoptosis through the mitochondrial pathway in human HepG2 cells. J Huazhong Univ Sci Technolog Med Sci, 2017,37(1):87–92
Edinger AL, Thompson CB. Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol, 2004,16(6):663–669
Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocr Rev, 1993,14(2):133–151
Peltomaki P. Role of DNA mismatch repair defects in the pathogenesis of human cancer. J Clin Oncol, 2003,21(6):1174–1179
Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell, 2011, 144 (5):646–674
Häcker G. The morphology of apoptosis. Cell Tissue Res, 2000,301(1):5–17
Evan GI, Vousden KH. Proliferation, cell cycle and apoptosis in cancer. Nature, 2001, 411 (6835):342–348
Wu BX, Zeidan YH, Hannun YA. Downregulation of neutral ceramidase by gemcitabine: Implications for cell cycle regulation. Biochim Biophys Acta, 2009,1791(8):730–739
Bentele M, Lavrik I, Ulrich M, et al. Mathematical modeling reveals threshold mechanism in CD95-induced apoptosis. J Cell Biol, 2004,166(6):839–851
Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol, 2007,35(6):495–516
Takahashi A, Masuda A, Sun M, et al. Oxidative stressinduced apoptosis is associated with alterations in mitochondrial caspase activity and Bcl-2-dependent alterations in mitochondrial pH. Brain Res Bull, 2004,62(6):497–504
Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science, 1998,281(5381):1322–1326
Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell, 1997,91(4):479–489
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This project was financially supported by the Natural Science Foundation of Hubei Province of China (No. 2017CFB517), the Talent Introduction Project of Hangzhou Medical College, Zhejiang Province, China (No. 2015B08), Open Project Fund of Hubei Province Key Laboratory of Occupational Hazard Identification and Control (No. OHIC2017G03), the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities (CZP17060) and National Natural Science Foundation of China (No. 81573239, No. 31671092 and No. 81773264).
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Wang, Q., Pan, Lh., Lin, L. et al. Essential Oil from Carpesium abrotanoides L. Induces Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells. CURR MED SCI 38, 1045–1053 (2018). https://doi.org/10.1007/s11596-018-1982-2
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DOI: https://doi.org/10.1007/s11596-018-1982-2