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Relationship between single nucleotide polymorphisms in −174G/C and −634C/G promoter region of interleukin-6 and prostate cancer

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Summary

The association between the single nucleotide polymorphisms (SNPs) in −174G/C and −634C/G of interleukin-6 (IL-6) promoter region and prostate cancer was examined in the population of Han people in Hubei region. TaqMan PCR was employed for the gene-typing of −174G/C and −634C/G in promoter region of IL-6 gene to compare the prostate cancer patients and normal controls in terms of genotype frequency, allele frequency and risk of prostate cancer. Enzyme-linked immunosorbent assay (ELISA) was used for the detection of IL-6 concentration in peripheral blood of the patients with prostate cancer and the relationship between the IL-6 level and the genotype was studied. Our results showed that in all the subjects, the genotype of genetic locus −174G/C was found to be GG and no CG and CC were observed. There was a significant difference in gene frequency of GG, CG and CC of −634C/G and allele frequency of G and C between prostate cancer patients and normal controls (P<0.05) and the gene frequency of GG+CG increased with the clinical stages and pathological grades of prostate cancer. The IL-6 level in GG+CG group was significantly higher than that in CC group. It was concluded that no SNP in −174G/C IL-6 promoter region was found in the population of Han people in Hubei region. The SNP in −634C/G was, to some extent, associated with the development and progression of prostate cancer. The population with GG+CG genetype has higher risk for prostate cancer.

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Authors and Affiliations

  1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Shixin Bao  (냼쫀탂), Weimin Yang  (퇮캪쏱), Siwei Zhou  (훜쯄캬) & Zhangqun Ye  (튶헂좺)

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  1. Shixin Bao  (냼쫀탂)
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  2. Weimin Yang  (퇮캪쏱)
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  3. Siwei Zhou  (훜쯄캬)
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  4. Zhangqun Ye  (튶헂좺)
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Shixin BAO, male, born in 1974, Doctoral Candidate

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Bao, S., Yang, W., Zhou, S. et al. Relationship between single nucleotide polymorphisms in −174G/C and −634C/G promoter region of interleukin-6 and prostate cancer. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 693–696 (2008). https://doi.org/10.1007/s11596-008-0618-3

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  • DOI: https://doi.org/10.1007/s11596-008-0618-3

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