Summary
In order to investigate the expression of Th1 and Th2 cytokines in the vaginal candidiasis caused by Candida, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C. albicans after the animals were pretreated with estradiol. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-2, IL-4, IL-10 and TGF-β1 in the vagina in the mice of different groups at different time points after the beginning of the experiment. The average expression level of IL-2 mRNA in group D (estrogen-treated mice) was significantly higher than that in groups H (estrogen-untreated mice) and I (control group) on the day 2. The average expression level of IL-4 mRNA in group D was significantly higher than that in groups I and H on the day 5. The average expression level of IL-10 mRNA in group D was significantly higher than that in groups H and I from day 7 to 11. The average expression level of TGF-β1 mRNA in group D was significantly higher than that in groups H and I at all time points. It was concludes that the high-level expression of IL-2 mRNA during early infection was associated with clearance of mucosal C. albicans, and the high-level expression of IL-10 mRNA during late stage of the infection was related to susceptibility to infection. TGF-β1 may play a predominant role when the virtual absence of changes in other Th-type cytokines during infection.
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Fidel P L Jr, Wolf N A, KuKuruga M A. T lymphocytes in the murine vaginal mucosa are phenotypically distinct from those in the periphery. Infect Immun, 1996,64:3793–3799
Fidel P L Jr, Lynch M E, Redondo-Lopez V et al. Systemic cell-mediated immune reactivity in women with recurrent vulvovaginal candidiasis (RVVC). J Infect Dis, 1993,168:1458–1465
Fidel P L Jr, Lynch M E, Conaway D H et al. Mice immunized by primary vaginal C. albicans infection develop acquired vaginal mucosal immunity. Infect Immun, 1995,63:547–553
Fidel P L Jr, Lynch M E, Sobel J D. Effects of preinduced Candida-specific systemic cell-mediated immunity on experimental vaginal candidiasis. Infect Immun, 1994,62:1032–1038
Romani L, Puccet T I P, Bistoni F. Biological role of Th cell subsets in candidiasis. In: Romagnani S, ed. Th1 and Th2 cells in Health and Disease. Farmington, CT: Karger, 1996.114–137
Cenci E, Mencacci A, Spaccapelo R et al. T helper cell type 1 (Th1)-and Th2-like responses are present in mice with gastric candidiasis but protective immunity is associated with Th1 development. J Infect Dis, 1995,171:1279–1288
Macher A M. The pathology of AIDS. Public Health Report, 1988,103:246–254
Clift R A. Candidiasis in the transplant patient. Am J Med, 1984,77(Suppl. 4D):34–38
Klein R S, Harris C A, Small C B et al. Oral candidiasis in high-risk patients as the initial manifestation of the acquired immunodeficiency syndrome. N Engl J Med, 1984,311:354–357
Taylor B N, Saavedra M, Fidel P L. Local Th1/Th2 cytokine production during experimental vaginal candidiasis: potential importance of transforming growth factor-β1. Med Mycol, 2000,38:419–431
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This project was supported by grants from the science Research Foundation of Health Department of Hubei Province, China (No. JXIB048) and the Janssen Research Foundation.
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Ouyang, W., Chen, S., Liu, Z. et al. Local Th1/Th2 cytokine expression in experimental murine vaginal candidiasis. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 352–355 (2008). https://doi.org/10.1007/s11596-008-0329-9
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DOI: https://doi.org/10.1007/s11596-008-0329-9