Abstract
Two new species of the lichen genus Lecaimmeria are described and illustrated from Pakistan, namely, Lecaimmeria asiatica and L. crispula. The former taxon is characterized by pale brown to dark brown thallus, up to 2-mm large areoles, flat to strongly convex apothecial disc, taller hymenium 120–150 µm, apically branched, not anastomosing paraphyses, large conidia 5–7 × 1.1–1.4 µm, with respect to ecology, and the absence of secondary metabolites, whereas L. crispula can be characterized by having a light brown to chocolate brown thallus, apothecia having flat to slightly concave discs with wavy margins that curl inward, a tall hymenium150–180 μm, branched and anastomosing paraphyses, large ascospores 25–33 × 12–17 μm, and absence of secondary metabolites. A phylogenetic analysis is provided based on nrITS and nrLSU sequences that support the separation of both novel species. Comparative analyses with related species of Lecaimmeria are provided to confirm the status of these species.
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Data availability
Samples analyzed during this study have been deposited in the LAH Herbarium, University of the Punjab, Lahore (https://vymaps.com/PK/LAH–Herbarium–Department–of–Botany–University–of–the–Punjab–Pakistan–334248940395482/). Sequences generated during this study have been deposited in NCBI, GenBank data repository (https://www.ncbi.nlm.nih.gov/genbank/). For list of accession numbers and voucher information, please see Appendix Table 2. All the alignments of phylogenies are available as supplementary information.
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Acknowledgements
We are extremely thankful to Dr. Kamran Habib for helping us with morphological description and M. Usman for helping us with sampling.
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All authors contributed to this study’s conception and design. Material preparation, data collection, and analysis were performed by Hafiza Simab Asghar, Iram Fayyaz, and Najam-ul-Sehar Afshan. The first draft of the manuscript was written by Hafiza Simab Asghar, and all authors commented on previous versions of the manuscript. Dr. Abdul Rehman Khan Niazi and Muhammad Nadeem also helped in the collection of the specimens from Pakistan that are used in this study. Naqibullah Oryakhil and Arooj Naseer helped in the collection from another site that is Afghanistan. Iram Fayyaz helped in the molecular analysis of this manuscript. Prof. Dr. Abdul Nasir Khalid supervised and validated the manuscript. All authors read and approved the final manuscript.
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Asghar, H.S., Nadeem, M., Oryakhil, N. et al. Two new species of Lecaimmeria (lichenized Ascomycota, Lecideaceae) from Asia. Mycol Progress 23, 13 (2024). https://doi.org/10.1007/s11557-024-01954-4
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DOI: https://doi.org/10.1007/s11557-024-01954-4