Abstract
In this study, two new species, namely Clitopilus highlandensis and C. subalbidus, are proposed based on morphological and molecular analyses. Clitopilus highlandensis differs from C. abprunulus by its greyish yellow and convex pileus, and larger basidiospores. Clitopilus subalbidus is similar to C. albidus but distinguished from the latter by its inflated hyphae in the lamellar trama and bigger basidiospores. The evidence from the phylogenetic tree constructed with RNA polymerase II second largest subunit (RPB2) further supports the morphological taxonomy. The phylogenetic tree, comprehensive descriptions, photographs, macro- and microscopic comparisons are presented.
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Data availability
In this study, DNA sequences have been deposited in GenBank. Specimens were placed at Herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (KUN-HKAS) and the Fungarium of the Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China (GDGM).
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Acknowledgements
The authors are very grateful to Drs. Bang Feng, Qing Cai, Gang Wu, Yan-Chun Li (Kunming Institute of Botany, Chinese Academy of Sciences), Xue-Tai Zhu (College of Life Sciences, Northwest Normal University), Mr. Xiang-Rong Zhong and Ms. Ya-Jun Hou for collecting and providing specimens. The authors thank the herbaria KUN-HKAS and GDGM for providing materials and pictures. The authors thank Mr. Zhi-Jia Gu for supplying scanning electron microscope (SEM) images. Dr. Timothy J. Baroni (State University of New York, US) is gratefully acknowledged for providing constructive comments.
Funding
This study was financially supported by the National Natural Science Foundation of China (Nos. 31670018, 31750001, 31970016) and the Biodiversity Survey and Assessment Project (No. 2019HJ2096001006) issued by Ministry of Ecology and Environment of the People’s Republic of China.
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Si-Peng Jian conceived, designed, and complete the experiments under the guidance of Zhu L. Yang. Si-Peng Jian and Xiang-Hua Wang made the line drawings. Si-Peng Jian wrote the manuscript, and Wang-Qiu Deng, Xiang-Hua Wang, and Zhu-Liang Yang revised it.
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Jian, SP., Wang, XH., Deng, WQ. et al. Clitopilus in southern China: two new species and comments on C. subscyphoides. Mycol Progress 22, 35 (2023). https://doi.org/10.1007/s11557-023-01885-6
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DOI: https://doi.org/10.1007/s11557-023-01885-6