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Rhizopogon confusus sp. nov., a correct name for a fungus previously recorded in Central Europe as the North American Rhizopogon salebrosus

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Abstract

Rhizopogon salebrosus, a common species found in western North America, has been recently considered an invasive species in Europe, based on ITS rDNA sequence records from ectomycorrhizal root tips. We collected in montane and submontane coniferous forests (both plantations and unmanaged forests) in the Czech Republic and Slovakia basidiocarps of a species with morphology similar to R. salebrosus. Analysis of sequences of ITS rDNA showed that our collections together with further records from Europe belonged into the R. salebrosus species group. In contrary, they formed a well-supported clade based on analysis of a gene for RNA polymerase II second largest subunit and one anonymous nuclear locus developed for better resolution in the R. salebrosus species group. Evidence from the three genes and distinct phenotypic characteristics show that the European collections represent a novel species within R. salebrosus species group that is described herein as R. confusus. Host affinity of R. confusus, its native presence in Europe and implications for the presumed invasion of R. salebrosus in Europe are discussed.

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Data availability

Sequences were submitted in GenBank and voucher specimens are deposited in herbaria.

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Acknowledgements

Special thanks are due to Jan Holec (PRM), Vladimír Antonín (BRNM), and Tereza Tejklová (HR) for arranging the loans and to Petra Veselá and Martina Vašutová for sharing their photos and data.

Funding

OK was supported by Institutional Support for Science and Research of the Ministry of Education, Youth and Sports and Ministry of Agriculture of the Czech Republic. VK was supported by the Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic (Project No. VEGA 1/0295/20).

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Charles University, Benátská 2, CZ-128 01, Prague, Czech Republic

    Ondřej Koukol & Nicholas J. Dowie

  2. Janova Ves 8, CZ-277 23, Kokořín, Czech Republic

    Slavomír Valda

  3. Nová 654, CZ-468 02, Rychnov u Jablonce nad Nisou, Czech Republic

    Jan Gaisler

  4. Department of Applied Ecology, Faculty of Ecology and Environmental Sciences, Technical University in Zvolen, T.G. Masaryka 24, SK-960 53, Zvolen, Slovak Republic

    Vladimír Kunca

Authors
  1. Ondřej Koukol
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  2. Slavomír Valda
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  3. Jan Gaisler
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  4. Vladimír Kunca
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  5. Nicholas J. Dowie
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Contributions

All authors contributed to the study conception and identification of collected material. Material preparation and collection were performed by S. Valda, J. Gaisler, and V. Kunca; molecular data were obtained and analysed by O. Koukol and interpreted by O. Koukol and N. Dowie. The first draft of the manuscript was written by O. Koukol and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ondřej Koukol.

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Section Editor: Zhu-Liang Yang

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Supplementary information

Online Resource 1:

Alignment of ITS rDNA in the .nex format. (NEX 44 kb)

Online Resource 2:

Alignment of RPB2 in the .nex format. (NEX 25 kb)

Online Resource 3:

Alignment of RAanl1 in the .nex format. (NEX 26 kb)

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Koukol, O., Valda, S., Gaisler, J. et al. Rhizopogon confusus sp. nov., a correct name for a fungus previously recorded in Central Europe as the North American Rhizopogon salebrosus. Mycol Progress 21, 49 (2022). https://doi.org/10.1007/s11557-022-01777-1

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