Abstract
In an investigation of Pythium species in China, two new species, P. agreste and P. wuhanense, were identified based on morphological characteristics and DNA sequence data. Pythium agreste has slightly inflated sporangia, oogonia encompassed by antheridia and antheridial stalks forming a very complicated knot, and plerotic oospores. It differs from the morphologically similar P. volutum which has inflated sporangia, bigger oogonia, and aplerotic oospores; from P. kashmirense which has contiguous inflated sporangia, catenulate oogonia and coiled or bent oogonial stalks; and from P. pectinolyticum which has catenulate oogonia and bigger oogonia and oospores. Pythium wuhanense can be differentiated from morphologically similar P. emineosum and P. paroecandrum by its possession of intercalary cylindrical to elongated sporangia and intercalary oogonia catenulate with sporangia and antheridia. Phylogenetic analyses showed that these two new species were clearly separated from morphologically similar Pythium species, based on the internal transcribed spacer (ITS) region and cytochrome C oxidase subunit I (COI) gene sequences using maximum parsimony and Bayesian methods. The two new species are described and illustrated in detail.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31270076, 31070434), the Guangxi Natural Science Foundation (No. 2011GXNSFA018078), the Plans for Construction of Scientific Topnotch and Innovation Team in Guangxi University, the Plans for Creative Training of Post-Graduates in Guangxi University (GXU11T31078), and the Central Public-interest Scientific Institution Basal Research Fund (2012–9). We thank Dr. Jo Taylor from Royal Botanic Garden Edinburgh for revising the manuscript and comments.
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Long, YY., Sun, X., Wei, JG. et al. Two new species, Pythium agreste and P. wuhanense, based on morphological characteristics and DNA sequence data. Mycol Progress 13, 145–155 (2014). https://doi.org/10.1007/s11557-013-0901-y
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DOI: https://doi.org/10.1007/s11557-013-0901-y