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Development and Evaluation of a Phospholipid-sterol-protein Membrane Resembling System

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Abstract

The development and evaluation of a phospholipid-sterol-protein membrane resembling system containing β-lactoglobulin (β-Lg) has been studied. Liposomes have been successfully employed for the protection, controlled release and site specific delivery of sensitive molecules, such as enzymes and vitamins. β-Lg (the major whey protein of ruminant species) was selected as model protein due to its ability to bind lipophilic molecules (i.e. phospholipids). Different techniques were used for the encapsulation studies (Differential Scanning Calorimetry, SDS polyacrylamide gel electrophoresis, Fourier Transform Infrared spectroscopy). Quantitation of encapsulation was estimated by Fluorescence spectroscopy. The stability study was carried out by turbidity measurements. The shape and size of the formulations prepared were assessed by Scanning Electron Microscopy and Dynamic Light Scattering. The developed system revealed a high encapsulation percentage of β-Lg, homogeneous and small shape and a very promising stability behavior. The characteristics obtained suggested that it is a very promising delivery system.

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References

  1. M.R. Mozafari, C.J. Reed, C. Rostron, C. Kocum, E. Piskin, Cell. Mol. Biol. Lett. 7(2), 243–244 (2002)

    Google Scholar 

  2. S.J. Park, S.G. Choi, E. Davaa, J.S. Park, Int. J. Pharm. 415(1–2), 267–272 (2011)

    Article  CAS  Google Scholar 

  3. M.R. Mozafari, K. Khosravi-Darani, G.G. Borazan, J. Cui, A. Pardakhty, S. Yurdugul, Int. J. Food Prop 11(4), 833–844 (2008)

    Article  CAS  Google Scholar 

  4. P. da Silva Malheiros, D.J. Daroit, A. Brandelli, Trends Food Sci. Technol. 21(6), 284–292 (2010)

    Article  Google Scholar 

  5. S.Y. Hwang, H.K. Kim, J. Choo, G.H. Seong, T.B.D. Hien, E.K. Lee, Colloids Surf. B 94, 296–303 (2012)

    Article  CAS  Google Scholar 

  6. S. Martins, B. Sarmento, D.C. Ferreira, E.B. Souto, Int. J. Nanomedicine 2(4), 595–607 (2007)

    CAS  Google Scholar 

  7. K.A. Edwards, A.J. Baeumner, Talanta 68(5), 1432–1441 (2006)

    Article  CAS  Google Scholar 

  8. A.S. Balkina, A.A. Selischeva, N.I. Larionova, Biochem. (Mosc.) Suppl. Ser. B 3(1), 48–53 (2009)

    Article  Google Scholar 

  9. D.I. Hǎdǎrugǎ, N.G. Hǎdǎrugǎ, C. Lǎzǎu, C. Raţiu, C. Crǎciun, I. Grozescu, Dig. J Nanomater. Biosci. 5(4), 919–925 (2010)

    Google Scholar 

  10. L. Zhang, S. Granick, Nano Lett. 6(4), 694–698 (2006)

    Article  CAS  Google Scholar 

  11. X. Zhang, N. Ge, T.A. Keiderling, Biochemistry 46(17), 5252–5260 (2007)

    Article  CAS  Google Scholar 

  12. X. Zhang, P. Tanner, A. Graff, C.G. Palivan, W. Meier, J. Polym. Sci. A Polym. 50(12), 2293–2318 (2012)

    Article  CAS  Google Scholar 

  13. L. Sawyer, in Advanced Dairy Chemistry, Volume 1A: Proteins: Basic Aspects, ed. by L. H. F. McSweeney, P.F. (Springer, 2013), pp. 211–259

  14. G. Kontopidis, C. Holt, L. Sawyer, J. Dairy Sci. 87(4), 785–796 (2004)

    Article  CAS  Google Scholar 

  15. G. Kontopidis, C. Holt, L. Sawyer, J. Mol. Biol. 318(4), 1043–1055 (2002)

    Article  CAS  Google Scholar 

  16. T. Lefèvre, M. Subirade, Int. J. Biol. Macromol. 28(1), 59–67 (2000)

    Article  Google Scholar 

  17. C.K. Kim, H.S. Chung, M.K. Lee, L.N. Choi, M.H. Kim, Int. J. Pharm. 183(2), 185–193 (1999)

    Article  CAS  Google Scholar 

  18. V. Ntimenou, S. Mourtas, E.V. Christodoulakis, M. Tsilimbaris, S.G. Antimisiaris, J Liposome Res. 16(4), 403–416 (2006)

    Article  CAS  Google Scholar 

  19. A. Badiee, M.R. Jaafari, A. Khamesipour et al., Exp. Parasitol. 121(4), 362–369 (2009)

    Article  CAS  Google Scholar 

  20. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual. (Cold Spring Harbor Laboratory Press, 2001)

  21. E.H. Gokce, E. Korkmaz, S. Tuncay-Tanriverdi et al., Int. J. Nanomedicine 7, 5109–5117 (2012)

    CAS  Google Scholar 

  22. M.M. Mady, M.W. Shafaa, E.R. Abbase, A.H. Fahium, Cell Biochem. Biophys. 62(3), 481–486 (2012)

    Article  CAS  Google Scholar 

  23. K. Dwiecki, P. Górnaś, A. Wilk, M. Nogala-Kałucka, K. Polewski, Cell. Mol. Biol. Lett. 12(1), 51–69 (2007)

    Article  CAS  Google Scholar 

  24. O. Gortzi, S. Lalas, I. Chinou, J. Tsaknis, J. Food Prot. 69(12), 2998–3005 (2006)

    Google Scholar 

  25. J.M. Rodríguez-Nogales, A.D. López, Int. Dairy J. 16(4), 354–360 (2006)

    Article  Google Scholar 

  26. X. Zhang, T.A. Keiderling, Biochemistry 45(27), 8444–8452 (2006)

    Article  CAS  Google Scholar 

  27. J. P. Colletier, B. Chaize, M. Winterhalter and D. Fournier, BMC Biotechnol 2 (2002)

  28. M. Zaru, S. Mourtas, P. Klepetsanis, A.M. Fadda, S.G. Antimisiaris, Eur. J. Pharm. Biopharm. 67(3), 655–666 (2007)

    Article  CAS  Google Scholar 

  29. A.M. Samuni, D.J.A. Crommelin, N.J. Zuidam, Y. Barenholz, J. Therm. Anal. Calorim. 51(1), 37–48 (1998)

    Article  CAS  Google Scholar 

  30. M. Bermúdez, E. Martinez, M. Mora, M.L. Sagristá, M.A. De Madariaga, Colloids Surf. A Physicochem. Eng. Asp. 158(1–2), 59–66 (1999)

    Article  Google Scholar 

  31. C. Heywang, M.S.P. Chazalet, M. Masson, A. Garnier-Suillerot, J. Bolard, Langmuir 12(26), 6459–6467 (1996)

    Article  Google Scholar 

  32. C. Bernsdorff, R. Reszka, R. Winter, J. Biomed. Mater. Res. 46(2), 141–149 (1999)

    Article  CAS  Google Scholar 

  33. N. Fa, S. Ronkart, A. Schanck et al., Chem. Phys. Lipids 144(1), 108–116 (2006)

    Article  CAS  Google Scholar 

  34. M. Fidorra, T. Heimburg, H.M. Seeger, BBA-Biomembr. 1788(3), 600–607 (2009)

    Article  CAS  Google Scholar 

  35. W. Cong, Q. Liu, Q. Liang, Y. Wang, G. Luo, Biophys. Chem. 143(3), 154–160 (2009)

    Article  CAS  Google Scholar 

  36. A. Junghans, C. Champagne, P. Cayot, C. Loupiac, I. Köper, Langmuir 27(6), 2709–2716 (2011)

    Article  CAS  Google Scholar 

  37. E. Chiavaro, M.T. Rodriguez-Estrada, A. Bendini, M. Rinaldi, L. Cerretani, J. Sci. Food Agric. 91(2), 198–206 (2011)

    Article  CAS  Google Scholar 

  38. C.C. Liolios, O. Gortzi, S. Lalas, J. Tsaknis, I. Chinou, Food Chem. 112(1), 77–83 (2009)

    Article  CAS  Google Scholar 

  39. A.S. Ulrich, Biosci. Rep. 22(2), 129–150 (2002)

    Article  CAS  Google Scholar 

  40. M. Rovoli, O. Gortzi, S. Lalas, G. Kontopidis, J. Liposome Res. 24(1), 74–81 (2014)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research has been co-financed by the European Union (European Social Fund - ESF) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: Heracleitus II. Investing in knowledge society through the European Social Fund.

We are grateful to Prof. Philippos J. Pomonis for the availability of the Scanning Electronic Microscope at his laboratory, Dr. Dimitrios Fatouros for his help with the DLS and Dr. Michael Siskos for his help with FTIR data interpretation. We also thank Prof. Lindsay Sawyer for valuable scientific discussion and help with the English.

Declaration of Interest

The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Department of Food Technology, Technological Educational Institution of Thessaly, Terma N. Temponera str., 43100, Karditsa, Greece

      Olga Gortzi & Stavros Lalas

    2. Laboratory of Biochemistry, Department of Veterinary Science, University of Thessaly, 224 Trikalon str., 43100, Karditsa, Greece

      Magdalini Rovoli & George Kontopidis

    Authors
    1. Olga Gortzi
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    2. Magdalini Rovoli
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    3. Stavros Lalas
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    4. George Kontopidis
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    Corresponding author

    Correspondence to George Kontopidis.

    Additional information

    Olga Gortzi and Magdalini Rovoli contributed equally to this work.

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    Gortzi, O., Rovoli, M., Lalas, S. et al. Development and Evaluation of a Phospholipid-sterol-protein Membrane Resembling System. Food Biophysics 10, 300–308 (2015). https://doi.org/10.1007/s11483-015-9390-7

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    • DOI: https://doi.org/10.1007/s11483-015-9390-7

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