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405 nm-Excited Fluorescence Spectra of the Juice of the Lemon (Citrus x Limon)

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Abstract

A 405 nm diode laser is used to excite fluorescence of juices of raw and ripe lemons. Emission bands appear approximately at 520 nm and 670 nm. Fluorescence intensity ratio F520/F670 is determined for the two stages. Variation in the fluorescence intensity ratio is observed during the process of ripening or growth of the fruit. Time-resolved spectra at this excitation wavelength reveal two decay times at both the stages at the emission wavelength of 520 nm, and two decay times at the raw stage and one decay time at the ripe stage at 670 nm.

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References

  1. O. Gulsen, M.L. Roose, J. Am. Soc. Hort. Sc. 126, 309 (2001)

    CAS  Google Scholar 

  2. www.nal.usda.gov/fnic/foodcomp/cgi-bin/list_nut_edit.pl (accessed on 10/11/2011)

  3. www.fineli.fi/food.php?foodid=11557&lang=en (accessed on 10/11/2011)

  4. P.K. Buah-Bassuah, H.M. von Bergmann, E.T. Tatchie, C.M. Steenkamp, Meas. Sci. Technol. 19, 025601 (2008)

    Article  Google Scholar 

  5. A. Gohain Barua, S. Hazarika, J. Sarmah Pathak, S. Manhas, Spectrosc. Lett. 43, 163 (2010)

    Article  Google Scholar 

  6. P.R. Borthakur, A. Gohain Barua, Natl. Acad. Sci. Lett. 35, 45 (2012)

    Article  Google Scholar 

  7. F. Morales, Z.G. Cerovic, I. Moya, Plant Physiol. 106, 127 (1994)

    CAS  Google Scholar 

  8. J.P. Wold, A. Verberg, F. Lundby, A.N. Nilsen, J. Moan, Int. Dairy J. 16, 1218 (2006)

    Article  CAS  Google Scholar 

  9. F. Guimet, J. Ferre, R. Boque, F.X. Rius, Anal. Chim. Acta 515, 75 (2004)

    Article  CAS  Google Scholar 

  10. D.K. Pedersen, L. Munck, S.B. Engelsen, J. Chemom. 16, 451 (2002)

    Article  CAS  Google Scholar 

  11. C. Buschmann, G. Langsdorf, H.K. Lichtenthaler, Photosynthetica 38, 483 (2000)

    Article  CAS  Google Scholar 

  12. M. Lang, F. Stober, H.K. Lichtenthaler, Radiat. Environ. Biophys. 30, 333 (1991)

    Article  CAS  Google Scholar 

  13. F. Morales, Z.G. Cerovic, I. Moya, Biochim. Biophys. Acta 1273, 251 (1996)

    Article  Google Scholar 

  14. Z.G. Cerovic, F. Morales, I. Moya, Biochim. Biophys. Acta 1188, 58 (1994)

    Article  CAS  Google Scholar 

  15. J.R. Lakowicz, Principles of fluorescence spectroscopy, 2nd edn. (Plenum, New York, 1999), pp. 63–64

    Book  Google Scholar 

  16. B. Myśliwa-Kurdziel, M.R. Amirjani, K. Strzalka, C. Sundqvist, Photochem. Photobiol. 78, 205 (2003)

    Article  Google Scholar 

  17. M. Jouy, C. Sironval, Planta 147, 127 (1997)

    Article  Google Scholar 

  18. J.B. Marder, V.I. Raskin, in Research in Photosynthesis: Proceedings of the IXth International Conference on Photosynthesis, Nagoya, Japan (August 30-September 4, 1992) vol. 1, ed. by N. Murata (Kluwer, Dordrecht, 1992) p. I.2.328

  19. A.T. Diplock, J.L. Charleux, G. Crozier-Willi, F.J. Kok, C. Rice-Evans, M. Roberfroid, W. Stahl, J. Vina-Ribes, British J. Nutr. 80(Suppl. 1), S77 (1998)

    Article  CAS  Google Scholar 

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Acknowledgement

Authors are grateful to Dr. B. Tanti, Department of Botany, Gauhati University, for some stimulating discussion.

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Authors and Affiliations

  1. Faculty of Science & Technology, The ICFAI University, Tripura, Kamalghat, Sadar, West Tripura, 799210, India

    Priyangshu Rana Borthakur

  2. Department of Physics, Darang College, Tezpur, 784001, India

    Simanta Hazarika

  3. LBMA Division, Raja Ramanna Center for Advanced Technology, Indore, 452013, India

    Pradeep Kumar Gupta

  4. Department of Physics, Gauhati University, Guwahati, 781014, India

    Anurup Gohain Barua

Authors
  1. Priyangshu Rana Borthakur
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  2. Simanta Hazarika
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  3. Pradeep Kumar Gupta
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  4. Anurup Gohain Barua
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Correspondence to Anurup Gohain Barua.

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Borthakur, P.R., Hazarika, S., Gupta, P.K. et al. 405 nm-Excited Fluorescence Spectra of the Juice of the Lemon (Citrus x Limon). Food Biophysics 8, 297–301 (2013). https://doi.org/10.1007/s11483-013-9304-5

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  • DOI: https://doi.org/10.1007/s11483-013-9304-5

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