Skip to main content
SpringerLink
Log in

MbCO Embedded in Trehalosyldextrin Matrices: Thermal Effects and Protein–Matrix Coupling

  • Special Issue Article
  • Published:
Food Biophysics Aims and scope Submit manuscript

Abstract

Saccharide-based biopreservation is widely studied because of its scientific importance and possible technological outcomes for food and pharmaceutical industries. Ternary protein/saccharide/water systems have been extensively exploited to model the characteristics of the in vivo biopreservation process. A tight, water dependent, protein–matrix coupling has been shown to occur in various simple saccharide amorphous matrices, which is stronger in trehalose. The efficiency as bioprotectant of trehalose has been ascribed to this tight coupling, since the appearance of damages on biological structures will more involve structural variations of the surrounding matrix. Here we present, as an applicative follow-up of this research line, a Fourier transform infrared study on protein–matrix coupling in commercial maltodextrins and trehalosyldextrins solid amorphous systems, with carboxymyoglobin embedded, and compare the results with analogous system containing trehalose and maltose, previously reported. Results point out that trehalosyldextrins are useful candidates as protecting agents, even though with an efficiency lower than trehalose, and could be used when the rheological properties of relative long-chain oligosaccharides are needed. However, it appears that a substantial improvement could be obtained by removal of the small fraction of glucose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. L.M. Crowe, Comp. Biochem. Physiol. A 132, 505 (2002)

    Article  Google Scholar 

  2. J.H. Crowe, Adv. Exp. Med. Biol. 594, 143 (2007)

    Article  Google Scholar 

  3. B. Roser, Trends Food Sci. Technol. 7, 166 (1991)

    Article  Google Scholar 

  4. L.I. Giannola, V. De Caro, G. Giandalia, M.G. Siragusa, L. Cordone, J. Ocular Pharmacol. Ther. 24, 186 (2008)

    Article  CAS  Google Scholar 

  5. N. Guo, I. Puhlev, D.R. Brown, J. Mansbridge, F. Levine, Nat. Biotechnol. 18, 168 (2000)

    Article  CAS  Google Scholar 

  6. C.L.A. Paiva, A.D. Panek, Biotechnol. Annu. Rev. 2, 293 (1996)

    Article  CAS  Google Scholar 

  7. J.F. Carpenter, J.H. Crowe, Biochemistry 28, 3916 (1989)

    Article  CAS  Google Scholar 

  8. P.S. Belton, A.M. Gil, Biopolymers 34, 957 (1994)

    Article  CAS  Google Scholar 

  9. J.G. Sampedro, S. Uribe, Mol. Cell Biochem. 256, 319 (2004)

    Article  Google Scholar 

  10. A. Hédoux, J.F. Willart, R. Ionov, F. Affouard, Y. Guinet, L. Paccou, A. Lerbret, M. Descamps, J. Phys. Chem. B 110, 22886 (2006)

    Article  Google Scholar 

  11. S. Magazù, G. Maisano, F. Migliardo, C. Mondelli, Biophys. J. 86, 3241 (2004)

    Article  Google Scholar 

  12. A. Cesàro, O. De Giacomo, F. Sussich, Food Chem. 106, 1318 (2008)

    Article  Google Scholar 

  13. F. Sussich, C. Skopec, J. Brady, A. Cesàro, Carbohydr. Res. 334, 165 (2001)

    Article  CAS  Google Scholar 

  14. A. Cesàro, Nat. Mater. 5, 593 (2006)

    Article  Google Scholar 

  15. S.J. Hagen, J. Hofrichter, W.A. Eaton, Science 269, 959 (1995)

    Article  CAS  Google Scholar 

  16. L. Cordone, G. Cottone, S. Giuffrida, G. Palazzo, G. Venturoli, C. Viappiani, BBA-Proteins Proteomics 1749, 252 (2005)

    Article  CAS  Google Scholar 

  17. S. Giuffrida, G. Cottone, L. Cordone, Biophys. J. 91, 968 (2006)

    Article  CAS  Google Scholar 

  18. L. Cordone, G. Cottone, S. Giuffrida, J. Phys.-Condens. Matter 19, 205110 (2007)

    Article  Google Scholar 

  19. L. Cordone, G. Cottone, S. Giuffrida, F. Librizzi, Chem. Phys. 345, 275 (2008)

    Article  CAS  Google Scholar 

  20. G. Cottone, L. Cordone, G. Ciccotti, Biophys. J. 80, 931 (2001)

    Article  CAS  Google Scholar 

  21. F. Francia, G. Palazzo, A. Mallardi, L. Cordone, G. Venturoli, Biochim. Biophys. Acta-Bioenerg. 1658, 50 (2004)

    Article  CAS  Google Scholar 

  22. G. Cottone, G. Ciccotti, L. Cordone, J. Chem. Phys. 117, 9862 (2002)

    Article  CAS  Google Scholar 

  23. G. Bellavia, L. Cordone, A. Cupane, J. Therm. Anal. Calorim. 95, 699 (2009)

    Article  CAS  Google Scholar 

  24. G. Bellavia, G. Cottone, S. Giuffrida, A. Cupane, L. Cordone, J. Phys. Chem. B 113, 11543 (2009)

    Article  CAS  Google Scholar 

  25. U. Heugen, G. Schwaab, E. Bründermann, M. Heyden, X. Yu, D.M. Leitner, M. Havenith, Proc. Natl. Acad. Sci. U. S. A. 103, 12301 (2006)

    Article  CAS  Google Scholar 

  26. M. Heyden, E. Bründermann, U. Heugen, G. Niehues, D.M. Leitner, M. Havenith, J. Am. Chem. Soc. 130, 5773 (2008)

    Article  CAS  Google Scholar 

  27. A. Longo, S. Giuffrida, G. Cottone, L. Cordone, Phys. Chem. Chem. Phys. 12, 6852 (2010)

    Article  CAS  Google Scholar 

  28. S. Giuffrida, G. Cottone, F. Librizzi, L. Cordone, J. Phys. Chem. B 107, 13211 (2003)

    Article  CAS  Google Scholar 

  29. S. Giuffrida, G. Cottone, L. Cordone, J. Phys. Chem. B 108, 15415 (2004)

    Article  CAS  Google Scholar 

  30. H. Frauenfelder, F. Parak, R.D. Young, Annu. Rev. Biophys. Chem. 17, 451 (1988)

    Article  CAS  Google Scholar 

  31. D. Eisenberg, W. Kauzmann, The Structure and Properties of Water (Oxford University Press, London, 1969)

    Google Scholar 

  32. S. Giuffrida, G. Cottone, E. Vitrano, L. Cordone, J. Non-Cryst. Solids (2010). doi:10.1016/j.jnoncrysol.2010.06.042

    Google Scholar 

  33. S.D. Allison, T.W. Randolph, M.C. Manning, K. Middleton, A. Davis, J.F. Carpenter, Arch. Biochem. Biophys. 358, 171 (1998)

    Article  CAS  Google Scholar 

  34. J.H. Crowe, J.F. Carpenter, L.M. Crowe, Annu. Rev. Physiol. 60, 73 (1998)

    Article  CAS  Google Scholar 

  35. J. Ubbink, M.I. Giardiello, H.J. Limbach, Biomacromolecules 8, 2862 (2007)

    Article  CAS  Google Scholar 

  36. S. Townrow, D. Kilburn, A. Alam, J. Ubbink, J. Phys. Chem. B 111, 12643 (2007)

    Article  CAS  Google Scholar 

  37. K. Imamura, T. Yokoyama, A. Fukushima, M. Kinuhata, K. Nakanishi, J. Pharm. Sci. 99, 4669 (2010)

    Article  CAS  Google Scholar 

  38. I. Di Lernia, C. Schiraldi, M. Generoso, M. De Rosa, Extremophiles 6, 341 (2002)

    Article  Google Scholar 

  39. C. Schiraldi, I. Di Lernia, M. Giuliano, M. Generoso, A. D’Agostino, M. De Rosa, J. Ind. Microbiol. Biotechnol. 30, 302 (2003)

    Article  CAS  Google Scholar 

  40. D. Cimini, M. De Rosa, A. Panariello, V. Morelli, C. Schiraldi, J. Ind. Microbiol. Biotechnol. 35, 1079 (2008)

    Article  CAS  Google Scholar 

  41. H. Kimoto–Nira, K. Mizumachi, M. Nomura, M. Kobayashi, Y. Fujita, T. Okamoto, I. Suzuki, N.M. Tsuji, J. Kurisaki, S. Ohmomo, Jpn. Agric. Res. Q. 41, 181 (2007)

    Google Scholar 

  42. M. Kato, Y. Miura, M. Kettoku, K. Shindo, A. Iwamatsu, Biosci. Biotechnol. Biochem. 60, 921 (1999)

    Article  Google Scholar 

  43. A. Lerbret, P. Bordat, F. Affouard, M. Descamps, F. Migliardo, J. Phys. Chem. B 109, 11046 (2005)

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Rosario Troia

    Present address: Medical systems Italia, General Electric Ltd., Rome, Italy

Authors and Affiliations

  1. Dipartimento di Scienze Fisiche ed Astronomiche and CNISM, Università degli Studi di Palermo, via Archirafi 36, 90123, Palermo, Italy

    Sergio Giuffrida, Rosario Troia & Lorenzo Cordone

  2. Dipartimento di Medicina Sperimentale, Sezione di Biotecnologia e Biologia Molecolare, Seconda Università di Napoli, via De Crecchio 7, 80138, Napoli, Italy

    Chiara Schiraldi, Antonella D’Agostino & Mario De Rosa

Authors
  1. Sergio Giuffrida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rosario Troia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chiara Schiraldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Antonella D’Agostino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mario De Rosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lorenzo Cordone
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sergio Giuffrida.

Additional information

This work was supported by the MIUR grant Piano Nazionale Ricerca per le Biotecnologie Avanzate II—Tema 6 “Biocatalisi”.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Giuffrida, S., Troia, R., Schiraldi, C. et al. MbCO Embedded in Trehalosyldextrin Matrices: Thermal Effects and Protein–Matrix Coupling. Food Biophysics 6, 217–226 (2011). https://doi.org/10.1007/s11483-010-9197-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11483-010-9197-5

Keywords

Search

Navigation