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Genetic diversity, geographic differentiation and evolutionary relationship among ecotypes of Glycine max and G. soja in China

  • Articles / Crop Germplasm Resources
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Chinese Science Bulletin

Abstract

The knowledge of origin and evolution of cultivated soybeans is one of the basic issues in both biology and agronomy of the crop. In order to investigate the nuclear and cytoplasmic genetic diversity, geographic differentiation and genetic relationship among geographic ecotypes of cultivated (Glycine max) and wild (G. soja) soybeans, the allelic profiles at 60 nuclear simple-sequence repeat (nuSSR) loci and 11 chloroplastic SSR (cpSSR) loci evenly distributed on whole genome of 393 landraces and 196 wild accessions from nation-wide growing areas in China were analyzed. (i) The genetic diversity of the wild soybean was obviously larger than that of the cultivated soybean, with their nuSSR and cpSSR alleles as 1067 vs. 980 and 57 vs 44, respectively. Of the 980 nuclear alleles detected in the cultivated soybean, 377 new ones (38.5%) emerged, while of the 44 chloroplastic alleles in the cultivated soybean, seven new ones (15.9%) emerged after domestication. (ii) Among the cultivated geographic ecotypes, those from southern China, including South-Central China, Southwest China and South China possessed relatively great genetic diversity than those from northern China, while among the wild geographic ecotypes, the Middle and Lower Changjiang Valleys wild ecotype showed the highest genetic diversity. (iii) The analysis of molecular variance, association analysis between geographic grouping and molecular marker clustering and analysis of specific-present alleles of ecotypes demonstrated that the geographic differentiation of both cultivated and wild soybeans associated with their genetic differentiation, or in other words, had their relevant genetic bases. (iv) The cluster analysis of all accessions clearly showed that the wild accessions from Middle and Lower Changjiang Valleys and South-Central & Southwest China had relatively small genetic distances with all cultivated accessions. The UPGMA dendrogram among geographic ecotypes further showed that the genetic distances between all cultivated ecotypes and the Middle and Lower Changjiang Valleys wild ecotype were smaller than those with other wild ones, including their local wild counterparts. Therefore, it is inferred that the wild ancestors in southern China, especially those from Middle and Lower Changjiang Valleys might be the common ancestor of all the cultivated soybeans.

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Authors and Affiliations

  1. Soybean Research Institute of Nanjing Agricultural University, Nanjing, 210095, China

    ZiXiang Wen, TuanJie Zhao, YanLai Ding & JunYi Gai

  2. National Center for Soybean Improvement, Nanjing, 210095, China

    ZiXiang Wen, TuanJie Zhao, YanLai Ding & JunYi Gai

  3. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing, 210095, China

    ZiXiang Wen, TuanJie Zhao, YanLai Ding & JunYi Gai

Authors
  1. ZiXiang Wen
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  2. TuanJie Zhao
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  3. YanLai Ding
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  4. JunYi Gai
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Corresponding author

Correspondence to JunYi Gai.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 32671266), National Key Basic Research and Development Program of China (Grant Nos. 2006CB101708 and 2009CB118404), Key Projects in the National Science & Technology Pillar Program (Grant No. 2006BAD13B05-7), Special Public Sector Research of the Ministry of Agriculture (Grant No. 200803060) and Programme of Introducing Talents of Discipline to Universities (111 Project) (Grant No. B08025)

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Wen, Z., Zhao, T., Ding, Y. et al. Genetic diversity, geographic differentiation and evolutionary relationship among ecotypes of Glycine max and G. soja in China. Chin. Sci. Bull. 54, 4393–4403 (2009). https://doi.org/10.1007/s11434-009-0696-z

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  • DOI: https://doi.org/10.1007/s11434-009-0696-z

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