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PX domain and CD domain play different roles in localization and vacuolation of Sorting Nexin 10

  • Articles/Developmental Genetics
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Chinese Science Bulletin

Abstract

Sorting nexins (SNXs) are PX domain containing proteins and essential for intracellular protein sorting, trafficking and signal transduction. The PX domains of SNXs can bind to various phosphorelated phosphoinositides (PIs) and target the host proteins to endosomes. Recently, we have reported that overexpression of SNX10 in mammalian cells could induce giant vacuoles. In this study, we aimed to identify regions in SNX10 critical for the vacuolation activity. We found that both the PX domain and the CD1 region were essential for vacuolation. We provided evidence that the PX domain was able to specifically bind to PtdIns(3)P and target SNX10 to endosomes. A mutation in the β1 region of the PX domain (V15A) disrupted the PtdIns(3)P binding ability and the endosomal localization of SNX10. However, correct subcellular localization alone was not sufficient for SNX10 to induce vacuoles. We found that the CD1 region, which was not required for the localization, was indispensable for the vacuolation activity of SNX10. In summary, both the PX domain and the CD1 region are necessary for SNX10 to induce vacuoles but they play different roles in this process.

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Authors and Affiliations

  1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Dong Yao

  2. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510663, China

    Dong Yao, Bin Wu, BaoMing Qin & DuanQing Pei

Authors
  1. Dong Yao
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  2. Bin Wu
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  3. BaoMing Qin
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  4. DuanQing Pei
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Corresponding author

Correspondence to DuanQing Pei.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 30700410)

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Yao, D., Wu, B., Qin, B. et al. PX domain and CD domain play different roles in localization and vacuolation of Sorting Nexin 10. Chin. Sci. Bull. 54, 3965–3971 (2009). https://doi.org/10.1007/s11434-009-0529-0

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  • DOI: https://doi.org/10.1007/s11434-009-0529-0

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