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Apoptosis induced by NaYF4:Eu3+ nanoparticles in liver cells via mitochondria damage dependent pathway

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Abstract

As lanthanide-doped sodium yttrium flouride (NaYF4) nanoparticles have great potential in biomedical applications, their biosafety is important and has attracted significant attention. In the present work, three different sized NaYF4:Eu3+ nanoparticles have been prepared. Liver BRL 3A cell was used as a cell model to evaluate their biological effects. Cell viability and apoptosis assays were used to confirm the cytotoxicity induced by NaYF4:Eu3+ NPs. Apart from the elevated malondialdehyde (MDA), the decrease of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) activity indicated reactive oxygen species (ROS) generation, which were associated with oxidative damage. The decrease of mitochondrial membrane potential (MMP) value demonstrated the occurrence of mitochondria damage. Then, release of cytochrome c from mitochondria and activation of caspase-3 confirmed that NaYF4:Eu3+ NPs induced apoptosis was mitochondria damage-dependent.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (21271059, 31470961, 21603051, 21601046, 31500812), Science and Technology Research Project of Higher Education Institutions in Hebei Province (QN2015230, QN2015132), the Natural Science Foundation of Hebei Province (B2015201097, B2016201169), and the Science and Technology Support Program of Baoding (15ZF055).

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Authors and Affiliations

  1. College of Chemistry & Environmental Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, 071002, China

    Shaohan Zhang, Shizhu Chen, Chunyue Gao, Yi Jin, Guang Jia, Zhenhua Li, Dandan Liu, Xinjian Yang & Jinchao Zhang

  2. CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China

    Xingjie Liang

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  1. Shaohan Zhang
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  2. Shizhu Chen
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Correspondence to Xinjian Yang or Jinchao Zhang.

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Zhang, S., Chen, S., Gao, C. et al. Apoptosis induced by NaYF4:Eu3+ nanoparticles in liver cells via mitochondria damage dependent pathway. Sci. China Chem. 60, 122–129 (2017). https://doi.org/10.1007/s11426-016-0225-5

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  • DOI: https://doi.org/10.1007/s11426-016-0225-5

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