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Multi-segment linear gradient optimization strategy based on resolution map in HPLC

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Abstract

Based on the mechanism of chromatographic retention (the relationship between the retention of solute and the mobile phase conditions) and method of resolution map, several methods of optimizing multi-segment linear gradient elution conditions were proposed according to the different separation requirements of various samples. These methods were verified using literature data. Moreover, the advantages and disadvantages of these methods were compared. It was proved that the third method is a fast optimization method which is capable of separating all the components with relatively high resolution.

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Authors and Affiliations

  1. Max-Planck-Institut für Dynamik Komplexer Technischer System, Magdeburg, 39106, Germany

    Shan Yichu, Zhang Weibing & Andreas Seidel-Morgenstern

  2. National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116011, China

    Zhang Weibing, Zhao Ruihuan & Zhang Yukui

Authors
  1. Shan Yichu
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  2. Zhang Weibing
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  3. Andreas Seidel-Morgenstern
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  4. Zhao Ruihuan
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  5. Zhang Yukui
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Correspondence to Zhang Weibing.

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Shan, Y., Zhang, W., Seidel-Morgenstern, A. et al. Multi-segment linear gradient optimization strategy based on resolution map in HPLC. SCI CHINA SER B 49, 315–325 (2006). https://doi.org/10.1007/s11426-006-2004-y

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  • DOI: https://doi.org/10.1007/s11426-006-2004-y

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