Summary
The marine prostanoid clavulones were shown to exert cytotoxicity against several cancer cells. In the present study, we illustrate the pathways utilized by clavulone II to trigger apoptotic signaling in human acute promyelocytic leukemia HL-60 cells. Exposure of cells to clavulone II resulted in early induction of phosphatidylserine externalization, mitochondrial dysfunction, and alteration of the cell cycle. Down-regulated expression of cyclin D1 explained the effect of clavulone II on G1 phase arrest of the cell cycle. Clavulone II induced the disruption of mitochondrial membrane potential and activation of caspase-8, -9 and -3 in a time- and concentration-dependent manner. Furthermore, the effect of 3 μM clavulone II was accompanied by the up-regulation of Bax, down-regulation of Mcl-1, and cleavage of Bid. Taken together, it is suggested that low concentrations of clavulone II induce the antiproliferative effect through the down-regulation of cyclin D1 expression and G1 arrest of the cell cycle, while that of high concentration induce the apoptotic cell death via the modulation of members of caspases and Bcl-2 family proteins in HL-60 cells.
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References
I. Herr K.M. Debatin (2001) ArticleTitleCellular stress response and apoptosis in cancer therapy Blood 98 2603–2614 Occurrence Handle10.1182/blood.V98.9.2603 Occurrence Handle1:CAS:528:DC%2BD3MXotVegs7o%3D Occurrence Handle11675328
D.S. Straus C.K. Glass (2001) ArticleTitleCyclopentenone prostaglandins: new insights on biological activities and cellular targets Med. Res. Rev. 21 185–210 Occurrence Handle10.1002/med.1006 Occurrence Handle1:CAS:528:DC%2BD3MXjsV2mu7g%3D Occurrence Handle11301410
M. Negishi H. Katoh (2002) ArticleTitleCyclopentenone prostaglandin receptors Prostag. Other Lipid. Mediat. 68–69 611–617 Occurrence Handle10.1016/S0090-6980(02)00059-X
A. Honda Y. Yamamoto Y. Mori Y. Yamada H. Kikuchi (1985) ArticleTitleAntileukemic effect of coral-prostanoids clavulones from the stolonifer Clavularia viridis on human myeloid leukemia (HL-60) cells Biochem. Biophys. Res. Commun. 130 515–523 Occurrence Handle10.1016/0006-291X(85)90447-4 Occurrence Handle1:CAS:528:DyaL2MXltFGrt7o%3D Occurrence Handle2862864
A. Honda S. Hong Y. Yamada Y. Mori (1991) ArticleTitleEffect of marine coral prostanoids, clavulones, on spontaneous beating rate of cultured myocardial cells from fetal mouse hearts Res. Commun. Chem. Pathol. Pharmacol. 72 363–366
T. Bader Y. Yamada H. Ankel (1991) ArticleTitleAntiviral activity of the prostanoid clavulone II against vesicular stomatitis virus Antiviral. Res. 16 341–355 Occurrence Handle10.1016/0166-3542(91)90048-V Occurrence Handle1:CAS:528:DyaK38Xht1SntrY%3D Occurrence Handle1663734
T. Mosmann (1983) ArticleTitleRapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays J. Immunol. Methods 65 55–63 Occurrence Handle1:STN:280:BiuD1cbgsFE%3D Occurrence Handle6606682
Y.T. Huang S.C. Chueh C.M. Teng J.H. Guh (2004) ArticleTitleInvestigation of ouabain-induced anticancer effect in human androgen-independent prostate cancer PC-3 cells Biochem. Pharmacol. 67 727–733 Occurrence Handle10.1016/j.bcp.2003.10.013 Occurrence Handle1:CAS:528:DC%2BD2cXnvVCruw%3D%3D Occurrence Handle14757172
C.J. Sherr (1995) ArticleTitleD-type cyclins Trends Biochem. Sci. 20 187–190 Occurrence Handle10.1016/S0968-0004(00)89005-2 Occurrence Handle1:CAS:528:DyaK2MXlslGns70%3D Occurrence Handle7610482
A. Honda Y. Mori K. Iguchi Y. Yamada (1987) ArticleTitleAntiproliferative and cytotoxic effects of newly discovered halogenated coral prostanoids from the Japaness stolonifer Clavularia viridis on human myeloid leukemia cells in culture Mol. Pharmacol 32 530–535 Occurrence Handle1:CAS:528:DyaL1cXitFA%3D Occurrence Handle2890095
M. Fukushima T. Kato S. Narumiya Y. Mizushima H. Sasaki Y. Terashima Y. Nishiyama M.G. Santoro (1989) ArticleTitleProstaglandin A and J: antitumor and antiviral prostaglandins Adv. Prostag. Thromboxane. Leukot. Res. 19 415–418 Occurrence Handle1:CAS:528:DyaL1MXkvFSnsbs%3D
T. Kato M. Fukushima S. Kurozumi R. Noyori (1986) ArticleTitleAntitumor activity of Δ7-prostaglandin A1 and Δ12-prostaglandin J2 in vitro and in vivo Cancer Res. 46 3538–3542
H.S. Kim H. Rhim S.W. Jeong J.W. Kim I.K. Kim (2002) ArticleTitleInduction of apoptosis dependent on caspase activities and growth arrest in HL-60 cells by PGA2 Prostag. Other Lipid. Mediat. 70 169–183 Occurrence Handle10.1016/S0090-6980(02)00064-3 Occurrence Handle1:CAS:528:DC%2BD38XptVOqsbg%3D
I.K. Kim J.H. Lee H.W. Sohn H.S. Kim S.H. Kim (1993) ArticleTitleProstaglandin A2 and Δ12-prostaglandin J2 induce apoptosis in L1210 cells FEBS Lett. 321 209–214 Occurrence Handle10.1016/0014-5793(93)80110-G Occurrence Handle1:CAS:528:DyaK3sXktVWmur0%3D Occurrence Handle8477853
M. Gorospe Y. Liu Q. Xu F.J. Chrest N.J. Holbrook (1996) ArticleTitleInhibition of G1 cyclin-dependent kinase activity during growth arrest of human breast carcinoma cells by prostaglandin A2 Mol. Cell. Biol. 16 762–770 Occurrence Handle1:CAS:528:DyaK28XhtFOmtbc%3D Occurrence Handle8622677
N. Marui T. Sakai N. Hosokawa M. Yoshida A. Aoike K. Kawai H. Nishino M. Fukushima (1990) ArticleTitleN-myc suppression and cell cycle arrest at G1 phase by prostaglandins FEBS Lett. 270 15–18 Occurrence Handle10.1016/0014-5793(90)81224-C Occurrence Handle1:CAS:528:DyaK3cXmtVGgtL0%3D Occurrence Handle2226777
C.V. Finkielstein L.G. Chen J.L. Maller (2002) ArticleTitleA role for G1/S cyclin-dependent protein kinase in the apoptotic response to ionizing radiation J. Biol. Chem. 277 38476–38485 Occurrence Handle10.1074/jbc.M206184200 Occurrence Handle1:CAS:528:DC%2BD38Xns1Cnsbo%3D Occurrence Handle12176996
M. Benekli M.R. Baer H. Baumann M. Wetzler (2003) ArticleTitleSignal transducer and activator of transcription proteins in leukemias Blood 101 2940–2954 Occurrence Handle10.1182/blood-2002-04-1204 Occurrence Handle1:CAS:528:DC%2BD3sXjtFCqsbs%3D Occurrence Handle12480704
V. Calò M. Migliavacca V. Bazan M. Macaluso M. Buscemi N. Gebbia A. Russo (2003) ArticleTitleSTAT proteins: from normal control of cellular events to tumorigenesis J. Cell. Physiol. 197 157–168 Occurrence Handle10.1002/jcp.10364 Occurrence Handle14502555
J. Turkson R. Jove (2000) ArticleTitleSTAT proteins: novel molecular targets for cancer drug discovery Oncogene 19 6613–6626 Occurrence Handle10.1038/sj.onc.1204086 Occurrence Handle1:CAS:528:DC%2BD3MXhsF2rsro%3D Occurrence Handle11426647
K. Spiekermann S. Biethahn S. Wilde W. Hiddemann F. Alves (2001) ArticleTitleConstitutive activation of STAT transcription factors in acute myelogenous leukemia Eur. J. Haematol. 67 63–71 Occurrence Handle1:CAS:528:DC%2BD3MXos12ksrc%3D Occurrence Handle11722592
C.A. Vosseler W. Erl P.C. Weber (2003) ArticleTitleStructural requirements of cyclopentenone prostaglandins to induce endothelial cell apoptosis Biochem. Biophys. Res. Commun. 307 322–326 Occurrence Handle10.1016/S0006-291X(03)01204-X Occurrence Handle1:CAS:528:DC%2BD3sXlt1artbs%3D Occurrence Handle12859958
M. Kondo T. Oya-Ito T. Kumagai T. Osawa K. Uchida (2001) ArticleTitleCyclopentenone prostaglandins as potential inducers of intracellular oxidative stress J. Biol. Chem. 276 12076–12083 Occurrence Handle10.1074/jbc.M009630200 Occurrence Handle1:CAS:528:DC%2BD3MXjtFylt7g%3D Occurrence Handle11278531
A. Nencioni K. Lauber F. Grünebach L. Van Parijs C. Denzlinger S. Wesselborg P. Brossart (2003) ArticleTitleCyclopentenone prostaglandins induce lymphocyte apoptosis by activating the mitochondrial apoptosis pathway independent of external death receptor signaling J. Immunol. 171 5148–5156 Occurrence Handle1:CAS:528:DC%2BD3sXoslyjurs%3D Occurrence Handle14607914
N. Yamakawa-Karakida K. Sugita T. Inukai K. Goi M. Nakamura K. Uno H. Sato K. Kagami N. Barker S. Nakazawa (2002) ArticleTitleLigand activation of peroxisome proliferator-activated receptor γ induces apoptosis of leukemia cells by down-regulating the c-myc gene expression via blockade of the Tcf-4 activity Cell. Death. Diff. 9 513–526 Occurrence Handle10.1038/sj.cdd.4401000 Occurrence Handle1:CAS:528:DC%2BD38XktF2qsL4%3D
P.K.M. Kim R. Mahidhara D.W. Seol (2001) ArticleTitleThe role of caspase-8 in resistance to cancer chemotherapy Drug Resist. Updat. 4 293–296 Occurrence Handle10.1054/drup.2001.0223 Occurrence Handle1:CAS:528:DC%2BD38Xkt1Sjs7Y%3D Occurrence Handle11991682
S. Cory D.C.S. Huang J.M. Adams (2003) ArticleTitleThe Bcl-2 family: roles in cell survival and oncogenesis Oncogene 22 8590–8607 Occurrence Handle1:CAS:528:DC%2BD3sXptFOmtb0%3D Occurrence Handle14634621
R.J. Lutz (2000) ArticleTitleRole of the BH3 (Bcl-2 homology 3) domain in the regulation of apoptosis and Bcl-2-related proteins Biochem. Soc. Trans. 28 51–56 Occurrence Handle1:CAS:528:DC%2BD3cXhsVKqtr0%3D Occurrence Handle10816098
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Huang, YC., Guh, JH., Shen, YC. et al. Investigation of anticancer mechanism of clavulone II, a coral cyclopentenone prostaglandin analog, in human acute promyelocytic leukemia. J Biomed Sci 12, 335–345 (2005). https://doi.org/10.1007/s11373-005-3009-9
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DOI: https://doi.org/10.1007/s11373-005-3009-9