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Dynamics of serum exosome microRNA profile altered by chemically induced estropause and rescued by estrogen therapy in female mice

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Abstract

The decline in the ovarian reserve leads to menopause and reduced serum estrogens. MicroRNAs are small non-coding RNAs, which can regulate gene expression and be secreted by cells and trafficked in serum via exosomes. Serum miRNAs regulate tissue function and disease development. Therefore, the aim of this study was to identify miRNA profiles in serum exosomes of mice induced to estropause and treated with 17β-estradiol (E2). Female mice were divided into three groups including control (CTL), injected with 4-Vinylcyclohexene diepoxide (VCD), and injected with VCD plus E2 (VCD + E2). Estropause was confirmed by acyclicity and a significant reduction in the number of ovarian follicles (p < 0.05). Body mass gain during estropause was higher in VCD and VCD + E2 compared to CTL females (p = 0.02). Sequencing of miRNAs was performed from exosomes extracted from serum, and 402 miRNAs were detected. Eight miRNAs were differentially regulated between CTL and VCD groups, seven miRNAs regulated between CTL and VCD + E2 groups, and ten miRNAs regulated between VCD and VCD + E2 groups. Only miR-200a-3p and miR-200b-3p were up-regulated in both serum exosomes and ovarian tissue in both VCD groups, suggesting that these exosomal miRNAs could be associated with ovarian activity. In the hepatic tissue, only miR-370-3p (p = 0.02) was up-regulated in the VCD + E2 group, as observed in serum. Our results suggest that VCD-induced estropause and E2 replacement have an impact on the profile of serum exosomal miRNAs. The miR-200 family was increased in serum exosomes and ovarian tissue and may be a candidate biomarker of ovarian function.

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Funding

The authors are thankful for the funding support provided by CAPES, CNPq, and FAPERGS to A.S., the National Institute on Aging of the National Institutes of Health under Award Number R56AG074499 to M.M.M and R56AG069676, R56AG064075, RF1AG071762, R21AG072379, U01AG076928, R21DE032197 to H.Y.; the Department of Defense (W81XWH-18-PRARP AZ180098 to H.Y.); and the Ed and Ethel Moore Alzheimer’s Disease Research Program of the Florida Department of Health (22A17) to H.Y.

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Authors and Affiliations

  1. Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Bianka Machado Zanini, Bianca Machado de Avila, Driele Neske Garcia, Jéssica Damé Hense, Gabriel Barreto Veiga, Mariana Machado Barreto & Augusto Schneider

  2. College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA

    Sarah Ashiqueali

  3. College of Veterinary Medicine, Department of Veterinary Clinical and Life Sciences, Center for Integrated BioSystems, Utah State University, Logan, UT, USA

    Jeffrey B. Mason

  4. USF Center for Microbiome Research, and Department of Neurosurgery and Brain Repair, Microbiomes Institute, University of South Florida, Tampa, FL, USA

    Hariom Yadav

  5. Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL, USA

    Michal Masternak

  6. Department of Head and Neck Surgery, Poznan University of Medical Sciences, Poznan, Poland

    Michal Masternak

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  1. Bianka Machado Zanini
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Zanini, B.M., de Avila, B.M., Garcia, D.N. et al. Dynamics of serum exosome microRNA profile altered by chemically induced estropause and rescued by estrogen therapy in female mice. GeroScience (2024). https://doi.org/10.1007/s11357-024-01129-9

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