Abstract
The objective of this study is to assess the occurrence of intestinal parasites in Mediterranean pond turtle Mauremys leprosa leprosa collected from three contrasting environments in Morocco. Stool samples from 92 turtles were examined for parasite detection and enumeration. The identified intestinal parasites belong to helminths (oxyurids and ascarid) and protozoa (Entamoebidae). A total of 25 turtles (27.17%) were found to be infected by helminths and/or protozoan parasites. No adult form of these parasites was detected. Eggs of oxyurid and ascarid were detected in individuals of populations studied from Oued Ksob (23.07% and 30.76% of n = 13 turtles) and Oued Zat (34.14% and 24.39% of n = 41 turtles), respectively. For protozoa, Entamoeba cysts were present in turtles in Oued Ksob (15.38% of n = 13 turtles), Oued Zat (12.19% of n = 41 turtles), and Oued Tensift (5.26% of n = 38 turtles) localities. Oxyurid eggs showed the highest intensity at Oued Zat reaching 29.30 ± 59.59 eggs per gram (EPG), versus 12 ± 0.38 EPG for ascaris eggs in Oued Ksob. Entamoeba cysts were detected in lower levels with a maximum of 1.66 ± 1.50 cysts per gram (CPG), in Oued Zat. The prevalence of turtles eliminating eggs was statistically significant between localities for different parasite groups. This study reports for the first time a parasitological characterization of gastrointestinal parasites in wild populations of M. leprosa leprosa from contrasting environments, suggesting a relationship between turtles’ infestation and the quality of their habitat.
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This research was funded by the Hubert Curien Programme; PHC Maghreb N°19MAG50 / 41431WD.
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Material preparation, data collection, and analysis were performed by EML and OA. The first draft of the manuscript was written by EML and revised EH El M. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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All experimental procedures were performed with the approval of the Moroccan Ethic Committee for Animal Research, UIZ-FSAA/08.
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Laghzaoui, EM., Amahmid, O. & El Mouden, E.H. Enteric parasites in free-living Mediterranean pond turtle (Mauremys leprosa leprosa) in contrasted areas (natural vs polluted) from central-western of Morocco. Environ Sci Pollut Res 31, 25559–25566 (2024). https://doi.org/10.1007/s11356-024-32796-y
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DOI: https://doi.org/10.1007/s11356-024-32796-y