Abstract
As the economy rapidly develops, chemicals are widely produced and used. This has exacerbated the problems associated with environmental pollution, raising the need for efficient toxicological evaluation techniques to investigate the toxic effects and mechanisms of toxicity of environmental pollutants. The progress in the techniques of cell culture in three dimensions has resulted in the creation of models that are more relevant in terms of biology and physiology. This enables researchers to study organ development, toxicology, and drug screening. Adult stem cells (ASCs) and induced pluripotent stem cells (iPSCs) can be obtained from various mammalian tissues, including cancerous and healthy tissues. Such stem cells exhibit a significant level of tissue memory and ability to self-assemble. When cultivated in 3D in vitro environments, the resulting organoids demonstrate a remarkable capacity to recapitulate the cellular composition and function of organs in vivo. Recently, many tumors’ tissue-derived organoids have been widely used in research on tumor pathogenesis, drug development, precision medicine, and other fields, including those derived from colon cancer, cholangiocarcinoma, liver cancer, and gastric cancer. However, the application of organoid models for evaluating the toxicity of environmental pollutants is still in its infancy. This review introduces the characteristics of the toxicity responses of organoid models upon exposure to pollutants from the perspectives of organoid characteristics, tissue types, and their applications in toxicology; discusses the feasibility of using organoid models in evaluating the toxicity of pollutants; and provides a reference for future toxicological studies on environmental pollutants based on organoid models.
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Funding
This work was supported by the National Natural Science Foundation of China (42077430), the Natural Science Foundation of Jiangsu Province, China (BK20221325), the Qing Lan Project of Jiangsu Province, and the Special Science and Technology Innovation Program for Carbon Peak and Carbon Neutralization of Jiangsu Province (BE2022612). The figures of this work were drawn by Figdraw (www.figdraw.com).
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Yuanhang Zhang: writing—original draft, literature search, and having the idea for the article. Kai Liu: data curation and literature search. Huan He: supervision and conceptualization. Hui Xiao: data curation. Zhihong Fang: data collection. Xianxian Chen: investigation. Huiming Li: writing—review and editing and funding acquisition. All authors reviewed the manuscript.
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Zhang, Y., Liu, K., He, H. et al. Innovative explorations: unveiling the potential of organoids for investigating environmental pollutant exposure. Environ Sci Pollut Res 31, 16256–16273 (2024). https://doi.org/10.1007/s11356-024-32256-7
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DOI: https://doi.org/10.1007/s11356-024-32256-7