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Characteristics of plant growth–promoting rhizobacteria SCPG-7 and its effect on the growth of Capsicum annuum L.

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Abstract

The strain SCPG-7 was isolated from saline soil in a cotton field. It is confirmed that the strain SCPG-7 is Pseudomonas sp. by means of the analysis of its phenotypic features and 16S rRNA sequence. SCPG-7 was capable of dissolving mineral tri-calcium phosphate (Ca3(PO4)2) and tri-magnesium phosphate (Mg3(PO4)2). In contrast, no showing iron phosphate (FePO4) or aluminum phosphate (AlPO4) solubilizing activities were detected by this experimental approach. The ratio of the dissolved P diameter to the colony diameter was 1.86. To study the phosphate dissolving mechanisms of the strain, we analyzed the changes of the pH value, the soluble phosphate content, the concentration of alkaline phosphatase, and the production of organic acid in the insoluble phosphate liquid medium. 2-keto-D-gluconicacid, α-ketoglutaric acid, succinic acid, etc. were characterized by LC-MS/MS in NBRIP medium. The concentration of 2-keto-D-gluconicacid increased to 88.6 mg/L after being cultured for 216 h. The strain decreased the pH value of the medium from 7.4 to 4.7 and the released soluble phosphate up to 516 mg/L, which proved the production of organic acids and alkaline phosphatase to be mechanism for solubilizing P. Under low phosphorus stress, Pseudomonas global regulatory protein PhoB regulates the transcription of the alkaline phosphatase gene. IAA and siderophore were secreted by SCPG-7. After treatment with SCPG-7, the individual plant height and dry weight of pepper increased by 23.3 and 31.2%, respectively, compared to the control group. The results show that the strain SCPG-7 has the potential to convert insoluble inorganic phosphorus to plant-available phosphorus. It can enhance soil phosphorus release through biological pathways, thereby increasing crop yield, and providing germplasm resources for the development of biological fertilizers.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Funding

This work was supported by the National Natural Science Foundation of China (grant 21575089) and Scientific Research Foundation for Changjiang Scholars of Shihezi University (CJXZ201501)

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering/The Key Lab. for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People’s Republic of China

    Yajie Han & Bang-Ce Ye

  2. College of Life Science, Shihezi University, Shihezi, 832003, People’s Republic of China

    Yajie Han & Fulong Chen

  3. Analysis and Testing Center, Shihezi University, Shihezi, 832003, China

    Shengxue Liu

  4. Teachers College, Shihezi University, Shihezi, 832003, People’s Republic of China

    Xiaolin Deng

  5. College of Materials Science and Engineering, Nanjing Tech University, Nanjing, 211800, China

    Zhuang Miao

  6. School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an, 710048, China

    Zhansheng Wu

Authors
  1. Yajie Han
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  2. Shengxue Liu
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  3. Fulong Chen
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  4. Xiaolin Deng
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  5. Zhuang Miao
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  6. Zhansheng Wu
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  7. Bang-Ce Ye
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Contributions

Yajie Han conceived the study, designed the study, and collected the data. Shengxue Liu, Fulong Chen, Xiaolin Deng, Zhuang Miao, Zhansheng Wu, and Bang-Ce Ye analyzed the data and were involved in writing the manuscript.

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Correspondence to Zhansheng Wu or Bang-Ce Ye.

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Han, Y., Liu, S., Chen, F. et al. Characteristics of plant growth–promoting rhizobacteria SCPG-7 and its effect on the growth of Capsicum annuum L.. Environ Sci Pollut Res 28, 11323–11332 (2021). https://doi.org/10.1007/s11356-020-11388-6

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  • DOI: https://doi.org/10.1007/s11356-020-11388-6

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