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Effects of exposure to triphenyltin (TPT) contaminant on sperm activity in adulthood of Calomys laucha exposed through breastfeeding

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Abstract

Triphenyltin (TPT) is an organotin compound (OT), primarily used in agriculture and in the composition of antifouling paints for ships worldwide. Studies have showed its effects as an endocrine disrupter in several organisms by preventing enzymatic expression and causing reproductive toxicity. This study aimed to evaluate the effects of exposure to TPT, via breastfeeding, on reproductive physiology in the Calomys laucha species. The experimental design was compound of five groups, two controls and three with different doses of TPT. Moreover, females were exposed by gavage to the TPT for 20 days, from the 1st day postpartum to the 21st postnatal day (PND). Then, the pups were euthanized and the kinetics, organelles, and biochemistry of the sperm were evaluated. The results presented a reduction in total motility in the groups exposed to TPT. Regarding cellular organelles analysis, a loss in membrane integrity was evidenced; the functionality of mitochondria showed diminution followed by increased acrosome reaction. In conclusion, the TPT causes alteration of the reproductive parameters, decreasing the activity and sperm quality in individuals exposed in the breastfeeding phase.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Fisiológicas Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil

    Tiane Ferreira de Castro & Graciela Quintana Saalfeld

  2. Reprodução Animal Comparada- RAC, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil

    Antônio Sergio Varela Junior, Jessica Ribeiro Pereira & Elton Pinto Colares

  3. Programa de Pós-Graduação em Biotecnologia Industrial, Universidade Tiradentes, Aracaju, SE, Brazil

    Francine Ferreira Padilha & Daniela Droppa-Almeida

  4. Reprodução Animal - Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Diego Martins Pires & Carine Dahl Corcini

Authors
  1. Tiane Ferreira de Castro
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  2. Antônio Sergio Varela Junior
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  3. Francine Ferreira Padilha
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  4. Daniela Droppa-Almeida
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  5. Graciela Quintana Saalfeld
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  6. Diego Martins Pires
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  7. Jessica Ribeiro Pereira
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  8. Carine Dahl Corcini
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  9. Elton Pinto Colares
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Corresponding author

Correspondence to Daniela Droppa-Almeida.

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All procedures were carried out in accordance with applicable standards and in the normative resolutions and guidelines of the National Council for Control of Animal Experimentation, approved by the Ethics Committee on Animal Use (CEUA-FURG) under the number Pq017 / 2014.

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Responsible editor: Philippe Garrigues

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de Castro, T.F., Varela Junior, A.S., Padilha, F.F. et al. Effects of exposure to triphenyltin (TPT) contaminant on sperm activity in adulthood of Calomys laucha exposed through breastfeeding. Environ Sci Pollut Res 26, 8280–8288 (2019). https://doi.org/10.1007/s11356-019-04365-1

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  • DOI: https://doi.org/10.1007/s11356-019-04365-1

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