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Preliminary evaluation of BioFire FilmArray® Gastrointestinal Panel for the detection of noroviruses and other enteric viruses from wastewater and shellfish

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Abstract

The BioFire FilmArray® Gastrointestinal Panel was evaluated for the rapid detection of adenovirus, astrovirus, norovirus, rotavirus and sapovirus from influent and effluent wastewater and shellfish. The multiplex BioFire FilmArray® Gastrointestinal Panel compared well to singleplex qPCR/RT-qPCR methods for the detection of adenovirus, astrovirus, rotavirus and sapovirus from influent and effluent wastewater samples. However, the BioFire FilmArray® Gastrointestinal Panel showed poor performance for the detection of norovirus, significantly underestimating its presence in wastewater and shellfish samples when compared with the singleplex norovirus GI and GII RT-qPCR assays. Therefore, improvement on detection efficiency for norovirus from environmental and food samples is necessary before using results from the FilmArray® Gastrointestinal Panel to assess associated public health risks.

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Funding

This work was funded by the Ministry of Business, Innovation and Employment (MBIE) (Safe New Zealand Seafood, CAWX1317).

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Authors and Affiliations

  1. Institute of Environmental Science and Research Ltd (ESR), Kenepuru Science Centre, PO Box 50348, Porirua, 5240, New Zealand

    Pradip Gyawali, Dawn Croucher & Joanne Hewitt

Authors
  1. Pradip Gyawali
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  2. Dawn Croucher
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  3. Joanne Hewitt
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Correspondence to Pradip Gyawali.

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Responsible editor: Philippe Garrigues

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Gyawali, P., Croucher, D. & Hewitt, J. Preliminary evaluation of BioFire FilmArray® Gastrointestinal Panel for the detection of noroviruses and other enteric viruses from wastewater and shellfish. Environ Sci Pollut Res 25, 27657–27661 (2018). https://doi.org/10.1007/s11356-018-2869-2

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  • DOI: https://doi.org/10.1007/s11356-018-2869-2

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