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Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

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Abstract

Metal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals’ toxic effects observed.

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Acknowledgments

The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project “BioInd-Biotechnology and Bioengineering for improved Industrial and Agro-Food processes” (NORTE-07-0124-FEDER-000028), Co-funded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER. Manuela D. Machado gratefully acknowledges the post-doctoral grant from FCT (SFRH/BPD/72816/2010). Vanessa A. Mesquita gratefully acknowledges the grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors also thank to Doctor Rosane Freitas Schwan to offer the yeast strain and to Doctor Helena M.V.M. Soares, from the Faculty of Engineering of Porto University, for the use of analytical facilities (AAS with flame atomization and AAS with electrothermal atomization).

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Bioengineering Laboratory, Chemical Engineering Department, ISEP-School of Engineering, Polytechnic Institute of Porto, Rua Dr António Bernardino de Almeida, 431, 4200-072, Porto, Portugal

    Vanessa A. Mesquita, Manuela D. Machado & Eduardo V. Soares

  2. Department of Biology, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil

    Vanessa A. Mesquita & Cristina F. Silva

  3. CEB-Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal

    Manuela D. Machado & Eduardo V. Soares

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  1. Vanessa A. Mesquita
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  2. Manuela D. Machado
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  4. Eduardo V. Soares
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Correspondence to Eduardo V. Soares.

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Mesquita, V.A., Machado, M.D., Silva, C.F. et al. Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii . Environ Sci Pollut Res 22, 11127–11136 (2015). https://doi.org/10.1007/s11356-015-4326-9

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