Abstract
Purpose
The goal of this study was to develop a plasmid-based lux bio-reporter for use to obtain in vivo images of Brucella suis vaccine strain 2 (B.suis S2) infection with high resolution and good definition.
Procedures
The pBBR-lux (pBBR1MCS-2-lxCDABE) plasmid that carries the luxCDABE operon was introduced into B. suis S2 by electroporation yielding B. suis S2-lux. The spatial and temporal transit of B. suis S2 in mice and guinea pigs was monitored by bioluminescence imaging.
Results
The plasmid pBBR-lux is stable in vivo and does not appear to impact the virulence or growth of bacteria. This sensitive luciferase reporter could represent B. suis S2 survival in real time. B. suis S2 mainly colonized the lungs, liver, spleen, and uterus in mice and guinea pigs as demonstrated by bioluminescence imaging.
Conclusion
The plasmid-based lux bioreporter strategy can be used to obtain high resolution in vivo images of B. suis S2 infection in mice and guinea pigs.
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Acknowledgments
Financial support for this study came from the National Project of Infectious Diseases (contract no. 2012ZX10004-502).
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Xiwen Wang and Zhiping Li contributed equally to this work.
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Wang, X., Li, Z., Li, B. et al. Bioluminescence Imaging of Colonization and Clearance Dynamics of Brucella Suis Vaccine Strain S2 in Mice and Guinea Pigs. Mol Imaging Biol 18, 519–526 (2016). https://doi.org/10.1007/s11307-015-0925-6
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DOI: https://doi.org/10.1007/s11307-015-0925-6