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In Vivo Quantification of Tumor Receptor Binding Potential with Dual-Reporter Molecular Imaging

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Abstract

Purpose

Receptor availability represents a key component of current cancer management. However, no approaches have been adopted to do this clinically, and the current standard of care is invasive tissue biopsy. A dual-reporter methodology capable of quantifying available receptor binding potential of tumors in vivo within a clinically relevant time scale is presented.

Procedures

To test the methodology, a fluorescence imaging-based adaptation was validated against ex vivo and in vitro measures of epidermal growth factor receptor (EGFR) binding potential in four tumor lines in mice, each line expected to express a different level of EGFR.

Results

A strong correlation was observed between in vivo and ex vivo measures of binding potential for all tumor lines (r = 0.99, p < 0.01, slope = 1.80 ± 0.48, and intercept = −0.58 ± 0.84) and between in vivo and in vitro for the three lines expressing the least amount of EGFR (r = 0.99, p < 0.01, slope = 0.64 ± 0.32, and intercept = 0.47 ± 0.51).

Conclusions

By providing a fast and robust measure of receptor density in tumors, the presented methodology has powerful implications for improving choices in cancer intervention, evaluation, and monitoring, and can be scaled to the clinic with an imaging modality like SPECT.

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Acknowledgements

This research was funded by NIH grants P01CA84201, R01CA109558, and R01CA156177. K. M. T. was funded in part by a CIHR postdoctoral fellowship.

Conflicts of interest

The authors have no conflicts of interest.

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Authors and Affiliations

  1. Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA

    Kenneth M. Tichauer, Kimberley S. Samkoe, Kristian J. Sexton, Shannon K. Hextrum, Harold H. Yang, W. Spencer Klubben, Jason R. Gunn & Brian W. Pogue

  2. Department of Surgery, Dartmouth Medical School, Lebanon, NH, 03756, USA

    Kimberley S. Samkoe & Brian W. Pogue

  3. Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, 02114, USA

    Tayyaba Hasan & Brian W. Pogue

Authors
  1. Kenneth M. Tichauer
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  2. Kimberley S. Samkoe
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  3. Kristian J. Sexton
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  4. Shannon K. Hextrum
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  5. Harold H. Yang
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  6. W. Spencer Klubben
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  7. Jason R. Gunn
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  8. Tayyaba Hasan
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  9. Brian W. Pogue
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Corresponding authors

Correspondence to Kenneth M. Tichauer or Brian W. Pogue.

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Tichauer, K.M., Samkoe, K.S., Sexton, K.J. et al. In Vivo Quantification of Tumor Receptor Binding Potential with Dual-Reporter Molecular Imaging. Mol Imaging Biol 14, 584–592 (2012). https://doi.org/10.1007/s11307-011-0534-y

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