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Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers

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Abstract

Sequence-related amplified polymorphism (SRAP) markers were used to assess genetic relationships among 76 grape genotypes including Chinese indigenous and newly bred varieties, representatives of foreign grape varieties, and wild Vitis species. Nineteen informative primers were selected from 100 SRAP primer pairs due to their ability to produce clearly and repeatedly polymorphic and unambiguous bands among the varieties. A total of 228 bands were produced; 78.63% of them were polymorphic; the average polymorphism information content (PIC) is 0.76. Genetic relationships were obtained using Nei and Li similarity coefficients. Cluster analysis of SRAP markers through the unweighted pair-group method of arithmetic averages (UPGMA) analysis and principal coordinate analysis (PCoA) were largely consistent. The definition of clusters in the dendrogram and PCoA plot is the same and some degree of grouping by types of grape, ecogeographical origin, and taxonomic status of the varieties was revealed. Three main groups were found after cluster analysis, i.e., table grape of Vitis vinifera; table grape of Euro-America hybrid and wine grape of V. vinifera; wild Vitis species. Groupings indicated a divergence between the table and wine-type varieties of V. vinifera. The results showed that the wild Vitis species that originated from America and China could be clearly differentiated and Vitis hancockii is the most distant from the others of Asian Vitis species. The results also indicated that SRAP markers are informative and could distinguish bud sports of grape. The present analysis revealed that Chinese cultivated and wild grape germplasm are highly variable and have abundant genetic diversity.

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Acknowledgments

This work was financially supported by Natural Science Foundation of China (NSFC:30800742), Natural Science Research Program of the Education Department of Henan Province (2009B210003),Young Teacher Funding Program of Henan Higher School (2010GGJS-072) and National Technology System for Grape Industry of China (nycytx-30-zy-01).

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Authors and Affiliations

  1. College of Forestry, Henan University of Science and Technology, No.70 Tianjin Road, Jianxi District, Luoyang, 471003, Henan Province, People’s Republic of China

    Dalong Guo, Junyu Zhang, Guohai Zhang, Meng Li & Qian Zhang

  2. Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, East Hanghai Road, Zhengzhou, 450009, Henan Province, People’s Republic of China

    Chonghuai Liu

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  1. Dalong Guo
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  2. Junyu Zhang
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  3. Chonghuai Liu
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  4. Guohai Zhang
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  5. Meng Li
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Correspondence to Dalong Guo or Chonghuai Liu.

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Communicated by R. Velasco

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Guo, D., Zhang, J., Liu, C. et al. Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers. Tree Genetics & Genomes 8, 789–800 (2012). https://doi.org/10.1007/s11295-011-0464-5

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