Abstract
Black heart rot is a serious disease of apricot and it has been reported to be caused by Alternaria solani, around the world. The present research was designed to control this disastrous disease using zinc oxide nanoparticles (b-ZnO NPs). These NPs were synthesized in the filtrate of a useful bacterium (Bacillus safensis) and applied to control black heart rot of apricot. After synthesis, the reduction of b-ZnO NPs was confirmed by UV-visible spectroscopy, at 330 nm. Fourier transform infrared (FTIR) spectra ensured the presence of multiple functional groups (alcohols, phenols, carboxylic acids, nitro compounds and amines) on the surface of b-ZnO NPs. X-Ray diffraction (XRD) analysis elucidated their average size (18 nm) while scanning electron microscopy (SEM) micrograph described the spherical shape of b-ZnO NPs. The synthesized b-ZnO NPs were applied in four different concentrations (0.25 mg/ml, 0.50 mg/ml, 0.75 mg/ml, 1.0 mg/ml) under both in vitro and in vivo conditions. These NPs were very efficient in inhibiting mycelial growth (85.1%) of A. solani at 0.75 mg/ml concentration of NPs, in vitro. Same concentration also performed best, in vivo, and significantly reduced disease incidence (by 67%) on self-inoculated apricot fruit. Apart from this, application of b-ZnO NPs helped apricot fruit to maintain its quality under fungal-stress conditions. The decay of apricot fruit was reduced and they maintained greater firmness and higher weight. Moreover, b-ZnO NPs treated fruits controlled black heart rot disease by maintaining higher contents of ascorbic acid, soluble sugars and carotenoids. These b-ZnO NPs were produced in powder form for their easy carriage to the farmers’ fields.
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Acknowledgements
This work was financially supported by the university research fund (URF 2020-21), Quaid-i-Azam University, Islamabad.
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FLNU: executed the experiments, Formal analysis, and wrote the manuscript. ABUF; contributed to conceptualization, UH; Data curation, Formal Analysis. HS; contributed to writing – review & MA; editing. MA; helped in Validation, MN; Conceptualization, Investigation, Formal analysis. HA; contributed to the characterization of fungus, JA; Data curation, Conceptualization, Contributed in characterization of extracts, HJC; conceptualization, and review & editing. MFHM; helped in software, writing – review & editing, contribute to the conceptualization, Designed the experiments, Resources, and Supervision.
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Farhana, Farooq, A.B.U., Haroon, U. et al. Bacillus safensis filtrate-based ZnO nanoparticles control black heart rot disease of apricot fruits by maintaining its soluble sugars and carotenoids. World J Microbiol Biotechnol 40, 125 (2024). https://doi.org/10.1007/s11274-024-03944-w
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DOI: https://doi.org/10.1007/s11274-024-03944-w