Abstract
Butyrate-producing bacteria are a functionally important part of the intestinal tract flora, and the resulting butyric acid is essential for maintaining host intestinal health, regulating the immune system, and influencing energy metabolism. However, butyrate-producing bacteria have not been defined as a coherent phylogenetic group. They are primarily identified using primers for key genes in the butyrate-producing pathway, and their use has been limited to the Bacillota and Bacteroidetes phyla. To overcome this limitation, we developed functional gene primers able to identify butyrate-producing bacteria through the butyrate kinase gene, which encodes the enzyme involved in the final step of the butyrate-producing pathway. Genomes extracted from human and rat feces were used to amplify the target genes through PCR. The obtained sequences were analyzed using BLASTX to construct a developmental tree using the MEGA software. The newly designed butyrate kinase gene primers allowed to recognize a wider diversity of butyrate-producing bacteria than that recognized using currently available primers. Specifically, butyrate-producing bacteria from the Synergistota and Spirochaetota phyla were identified for the first time using these primers. Thus, the developed primers provide a more accurate method for researchers and doctors to identify potential butyrate-producing bacteria and deepen our understanding of butyrate-producing bacterial species.
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This study was supported by the National Natural Science Foundation of China (82160788), Jiangxi University of Traditional Chinese Medicine, First Level of Integrative Medicine.
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This study was supported by the National Natural Science Foundation of China (82160788) and the Top Discipline of Jiangxi Province, Discipline of Chinese and Western Integrative Medicine, Jiangxi University of Chinese Medicine (zxyylxk20220103).
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Meng, X., Shu, Q. Novel primers to identify a wider diversity of butyrate-producing bacteria. World J Microbiol Biotechnol 40, 76 (2024). https://doi.org/10.1007/s11274-023-03872-1
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DOI: https://doi.org/10.1007/s11274-023-03872-1