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The novel oleaginous bacterium Sphingomonas sp. EGY1 DSM 29616: a value added platform for renewable biodiesel

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Abstract

Oleaginous microorganisms are regarded as efficient, renewable cell factories for lipid biosynthesis, a biodiesel precursor, to overwhelm the cosmopolitan energy crisis with affordable investment capital costs. Present research highlights production and characterization of lipids by a newly isolated oleaginous bacterium, Sphingomonas sp. EGY1 DSM 29616 through an eco-friendly approach. Only sweet whey [42.1% (v/v)] in tap water was efficiently used as a growth medium and lipid production medium to encourage cell growth and trigger lipid accumulation simultaneously. Cultivation of Sphingomonas sp. EGY1 DSM 29616 in shake flasks resulted in the accumulation of 8.5 g L−1 lipids inside the cells after 36 h at 30 °C. Triglycerides of C16:C18 saturated and unsaturated fatty acids showed a similar pattern to tripalmitin or triolein; deduced from gas chromatography (GC), thin layer chromatography (TLC), and Matrix-assisted laser desorption/ionization time-of-flight-mass spectra analysis (MALDI-TOF-MS) analyses. Batch cultivation 2.5 L in a laboratory scale fermenter led to 13.8 g L−1 accumulated lipids after 34 h at 30 °C. Present data would underpin the potential of Sphingomonas sp. EGY1 DSM 29616 as a novel renewable cell factory for biosynthesis of biodiesel.

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Author contributions

NA conducted all of the experimental laboratory work. YE revised the whole manuscript, put the designs of some experiments especially scaling up experiment and discussed the whole laboratory data. AME wrote the whole manuscript drafting, carried out molecular identification of the bacterial isolate, analyzed, and discussed the whole laboratory data. AH carried out MALDI-TOF-MS experiment and discussed the whole laboratory data. All authors approved the final manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, 163 EL-Horreya Avenue, Alexandria, 21526, Egypt

    Nehad N. Amer, Amira M. Embaby & Ahmed Hussein

  2. Faculty of Science, Botany and Microbiology Department, Alexandria University, Moharam Baik, Alexandria, 21511, Egypt

    Yasser Elbahloul

  3. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA

    Ahmed Hussein

Authors
  1. Nehad N. Amer
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  2. Yasser Elbahloul
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  3. Amira M. Embaby
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  4. Ahmed Hussein
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Correspondence to Amira M. Embaby.

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Amer, N.N., Elbahloul, Y., Embaby, A.M. et al. The novel oleaginous bacterium Sphingomonas sp. EGY1 DSM 29616: a value added platform for renewable biodiesel. World J Microbiol Biotechnol 33, 145 (2017). https://doi.org/10.1007/s11274-017-2305-7

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  • DOI: https://doi.org/10.1007/s11274-017-2305-7

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