Abstract
This study assessed the efficiency of Scheffersomyces amazonensis UFMG-CM-Y493T, cultured in xylose-supplemented medium (YPX) and rice hull hydrolysate (RHH), to convert xylose to xylitol under moderate and severe oxygen limitation. The highest xylitol yields of 0.75 and 1.04 g g−1 in YPX and RHH, respectively, were obtained under severe oxygen limitation. However, volumetric productivity in RHH was ninefold decrease than that in YPX medium. The xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in the YPX cultures were strictly dependent on NADPH and NAD+ respectively, and were approximately 10% higher under severe oxygen limitation than under moderate oxygen limitation. This higher xylitol production observed under severe oxygen limitation can be attributed to the higher XR activity and shortage of the NAD+ needed by XDH. These results suggest that Sc. amazonensis UFMG-CM-Y493T is one of the greatest xylitol producers described to date and reveal its potential use in the biotechnological production of xylitol.
References
Almeida JRM, Modig T, Petersson A, Hähn-Hägerdal B, Lidén G, Gorwa-Grauslund MF (2007) Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J Chem Technol Biotechnol 82:340–349
Bruinenberg PM, de Bot PH, van Dijken JP, Scheffers WA (1984) NADH-linked aldose reductase: the key to anaerobic alcoholic fermentation of xylose by yeasts. Appl Microbiol Biotechnol 19:256–260
Cadete RM, Melo MA, Dussan KJ, Rodrigues RC, Silva SS, Zilli JE et al (2012a) Diversity and physiological characterization of d-xylose-fermenting yeasts isolated from the Brazilian Amazonian forest. PLoS ONE 7:e43135
Cadete RM, Melo MA, Lopes MR, Pereira GM, Zilli JE, Vital MJ et al (2012b) Candida amazonensis sp. nov., an ascomycetous yeast isolated from rotting wood in the Amazonian forest. Int J Syst Evol Microbiol 62:1438–1440
Cadete RM, Cheab MA, Santos RO, Safar SV, Zilli JE, Vital MJ et al (2015) Cyberlindnera xylosilytica sp. nov., a xylitol-producing yeast species isolated from lignocellulosic materials. Int J Syst Evol Microbiol 65:2968–2974
Cadete RM, de las Heras AM, Sandström AG, Ferreira C, Gírio F, Gorwa-Grauslund M-F et al (2016) Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae. Biotechnol Biofuels 9:167
da Cunha-Pereira F, Hickert LR, Sehnem NT, de Souza-Cruz PB, Rosa CA, Ayub MAZ (2011) Conversion of sugars present in rice hull hydrolysates into ethanol by Spathaspora arborariae, Saccharomyces cerevisiae, and their co-fermentations. Bioresour Technol 102:4218–4225
da Silva SS, Chandel AK (2012) d-Xylitol. Springer, Berlin
de Albuquerque TL, da Silva IJ, de Macedo GR, Rocha MVP (2014) Biotechnological production of xylitol from lignocellulosic wastes: a review. Process Biochem 49:1779–1789
Guamán-Burneo MC, Dussán KJ, Cadete RM, Cheab MA, Portero P, Carvajal-Barriga EJ et al (2015) Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis fa, sp. nov. Antonie Van Leeuwenhoek 108:919–931
Junyapate K, Jindamorakot S, Limtong S (2014) Yamadazyma ubonensis fa, sp. nov., a novel xylitol-producing yeast species isolated in Thailand. Antonie Van Leeuwenhoek 105:471–480
Oh DK, Kim SY (1998) Increase of xylitol yield by feeding xylose and glucose in Candida tropicalis. Appl Microbiol Biotechnol 50:419–425
Pal S, Mondal AK, Sahoo DK (2016) Molecular strategies for enhancing microbial production of xylitol. Process Biochem 51:809–819
Rafiqul ISM, Sakinah AM (2013) Processes for the production of xylitol—a review. Food Rev Int 29:127–156
Rao LV, Goli JK, Gentela J, Koti S (2016) Bioconversion of lignocellulosic biomass to xylitol: an overview. Bioresour Technol 213:299–310
Ur-Rehman S, Mushtaq Z, Zahoor T, Jamil A, Murtaza MA (2015) Xylitol: a review on bioproduction, application, health benefits, and related safety issues. Crit Rev Food Sci Nutr 55:1514–1528
Winkelhausen E, Kuzmanova S (1998) Microbial conversion of d-xylose to xylitol. J Ferment Bioeng 86:1–14
Yablochkova EN, Bolotnikova OI, Mikhailova NP, Nemova NN, Ginak AI (2003) Specific features of fermentation of d-xylose and d-glucose by xylose-assimilating yeasts. Appl Biochem Microbiol 39:265–269
Yablochkova EN, Bolotnikova OI, Mikhailova NP, Nemova NN, Ginak AI (2004) The activity of key enzymes in xylose-assimilating yeasts at different rates of oxygen transfer to the fermentation medium. Microbiology 73:129–133
Zhang F, Qiao D, Xu H, Liao C, Li S, Cao Y (2009) Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis. J Microbiol 47:351–357
Acknowledgements
RMC acknowledge the financial support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, PDEE, Process No. 4782-11-9). This work was co-funded by the European Commission in the framework of EU-Brazil Project ProEthanol2G ‘‘Integration of Biology and Engineering into an Economical and Energy-Efficient 2G Bioethanol Biorefinery’’ (FP7-251151), by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Process Nos. 551392/2010-0, 551245/2010-7 and 560715/2010-2), by the Financiadora de Estudos e Projetos (FINEP, Process No. 2084/07) and by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Cadete, R.M., Melo-Cheab, M.A., Viana, A.L. et al. The yeast Scheffersomyces amazonensis is an efficient xylitol producer. World J Microbiol Biotechnol 32, 207 (2016). https://doi.org/10.1007/s11274-016-2166-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11274-016-2166-5