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Sediment prokaryote communities in different sites of eutrophic Lake Taihu and their interactions with environmental factors

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Abstract

To investigate the temporal variation of the sediment prokaryote communities and their relation with the rapid increase of algae population in Taihu, a shallow eutrophic freshwater Lake, water and surface sediments were sampled from seven sites in different stages of algal bloom. The physicochemical characterization revealed positive correlations among the nutrient (N, P) parameters in the water and sediments, as well as TN/TP ratio 30.79 in average in water and 0.13 in sediments, demonstrating that P content was the limit factor for bloom in Taihu and sediment was an important nutrient resource for the water body. T-RFLP analysis of 16S rRNA genes revealed a diversity decrease of sediment prokaryotic communities along the bloom. The bacterial communities in sediments were more sensitive and shaped by the temporal changes, while archaea were more sensitive to the trophic level. The pyrosequencing data showed clear spatial and temporal changes in diversity of sediment bacteria. Betaproteobacteria was the most abundant group in all the samples, following by Delta-, Gama- and Alpha-proteobacteria, Acidobacteria, Chlorobi, Chloroflexi etc. At the genus level, Thiobacillus and Sulfuricurvum were the most dominant groups in the sediments, and the increase of Thiobacillus abundance in February might be used as bioindicator for the subsequent bloom. In addition, NO3 −-N was evidenced to be the main factor to regulate the bacterial community structure in the sediments. These results offered some novel and important data for the evaluation and predict the algal bloom in Taihu and can be reference for other shallow fresh water lakes.

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Acknowledgments

We are grateful to the TLLER for its assistance with sample collection. This work was supported by the National Natural Science Foundation of China (No. 31070105) and the China Geological Survey grant (No. 12120113015200). ETW was financially supported by the projects SIP 20120760 and 20130828 authorized by Instituto Politecnico Nacional, México.

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  1. State Key Laboratories for Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People’s Republic of China

    Nan Chen, Jin-Shui Yang, Jiang-Hang Qu, Hai-Feng Li, Wei-Jie Liu, Bao-Zhen Li & Hong-Li Yuan

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Mexico, DF, Mexico

    En Tao Wang

  3. Room 1061, Center of Life Science, China Agriculture University, No. 2 Yuanmingyuanxilu, Haidian District, Beijing, 100193, People’s Republic of China

    Hong-Li Yuan

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  1. Nan Chen
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Chen, N., Yang, JS., Qu, JH. et al. Sediment prokaryote communities in different sites of eutrophic Lake Taihu and their interactions with environmental factors. World J Microbiol Biotechnol 31, 883–896 (2015). https://doi.org/10.1007/s11274-015-1842-1

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