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Characterization of rumen bacterial strains isolated from enrichments of rumen content in the presence of propolis

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Abstract

Propolis presents many biological properties, including antibacterial activities, and has been proposed as an additive in ruminant nutrition. Twenty bacterial strains, previously isolated from enrichments of Brazilian cow rumen contents in the presence of different propolis extracts (LLOS), were characterized using phenotyping and 16S rRNA identification. Seven strains were assigned to Streptococcus sp., most likely S. bovis, and were all degrading starch. One amylolytic lactate-utilizing strain of Selenomonas ruminantium was also found. Two strains of Clostridium bifermentans were identified and showed proteolytic activity. Two strains were assigned to Mitsuokella jalaludinii and were saccharolytic. One strain belonged to a Bacillus species and seven strains were affiliated with Escherichia coli. All of the 20 strains were able to use many sugars, but none of them were able to degrade the polysaccharides carboxymethylcellulose and xylans. The effect of three propolis extracts (LLOS B1, C1 and C3) was tested on the in vitro growth of four representative isolates of S. bovis, E. coli, M. jalaludinii and C. bifermentans. The growth of S. bovis, E. coli and M. jalaludinii was not affected by the three propolis extracts at 1 mg ml−1. C. bifermentans growth was completely inhibited at this LLOS concentration, but this bacterium was partially resistant at lower concentrations. LLOS C3, with the lower concentration of phenolic compounds, was a little less inhibitory than B1 and C1 on this strain.

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Acknowledgments

The authors thank G. Andant and G. Lopes for excellent technical assistance and the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Empresa Brasileira de Pesquisa Agropecuária (Embrapa) for granting the scholarship to S.C. Aguiar (CNPq/Labex Embrapa 201720/2010-8).

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Authors and Affiliations

  1. Departamento de Zootecnia, Universidade Estadual de Maringá, Avenida Colombo 5790, Maringá, PR, 87020-900, Brazil

    Sílvia Cristina de Aguiar & Lucia Maria Zeoula

  2. INRA, Unité de Microbiologie UR454, Centre de Recherches de Clermont-Ferrand/Theix, 63122, Saint-Genes-Champanelle, France

    Sílvia Cristina de Aguiar & Evelyne Forano

  3. Departamento de Zootecnia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 km 380, Campus Universitário, Londrina, PR, 86057-970, Brazil

    Odimari Pricila Pires do Prado

  4. Ruminal Microbiology Laboratory, Embrapa Gado de Leite, Juiz de Fora, MG, Brazil

    Pedro Braga Arcuri

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  1. Sílvia Cristina de Aguiar
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  2. Lucia Maria Zeoula
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  3. Odimari Pricila Pires do Prado
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  4. Pedro Braga Arcuri
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  5. Evelyne Forano
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Correspondence to Evelyne Forano.

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de Aguiar, S.C., Zeoula, L.M., do Prado, O.P.P. et al. Characterization of rumen bacterial strains isolated from enrichments of rumen content in the presence of propolis. World J Microbiol Biotechnol 30, 2917–2926 (2014). https://doi.org/10.1007/s11274-014-1719-8

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