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Improved synchronous light scattering method for measuring baker’s yeast biomass using thickened suspensions

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Abstract

Measuring yeast biomass is important in the processes of microbial fermentations. It has been demonstrated that synchronous light scattering (SLS) signals could be applied for the quantification of model bioparticles such as Saccharomyces cerevisiae. In this study, an improved synchronous light scattering method was developed for yeast biomass estimation. The settlement of yeast cells during SLS signals measuring process was studied, and hydrolysis anionic polyacrylamide was added into yeast suspensions to increase the stability of the cells in liquid environment. By simultaneously scanning both the excitation and emission monochromators of a common spectrofluorometer with same starting excitation and emission wavelength (namely, ∆λ = 0), the SLS intensity was found to be proportional to the yeast concentration in the range from 0 to 4.9 × 106 cell/mL (R 2 = 0.9907), the detection limit is 8.1 × 103 cell/mL. The developed method exhibited good stability and sensitivity in the recovery test and growth curve drawing process, demonstrating the potential of the method in practical application of biomass estimation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21176125), the Science Research Project of the Ministry of Education of Heilongjiang Province of China (2012TD012, 12511Z030), and the National Natural Science Foundation of Heilongjiang Province (B201114, YJSCX2012-385HLJ).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, 161006, China

    Zhen Wang, Xiangfeng Guo & Lihua Jia

  2. Key Laboratory of Fine Chemicals (Qiqihar University), College of Heilongjiang Province, Qiqihar, 161006, China

    Xiangfeng Guo & Ying Ding

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  1. Zhen Wang
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  2. Xiangfeng Guo
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  3. Lihua Jia
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  4. Ying Ding
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Correspondence to Xiangfeng Guo or Lihua Jia.

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Wang, Z., Guo, X., Jia, L. et al. Improved synchronous light scattering method for measuring baker’s yeast biomass using thickened suspensions. World J Microbiol Biotechnol 29, 1531–1536 (2013). https://doi.org/10.1007/s11274-013-1315-3

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  • DOI: https://doi.org/10.1007/s11274-013-1315-3

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