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Rapid detection of methicillin resistance in Staphylococcus aureus isolates; evaluation of colorimetric Quicolor ES agar and determination of breakpoint inhibition zone diameters of cefoxitin

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Abstract

In this study, it was aimed to evaluate colorimetric Quicolor ES agar for the rapid detection of methicillin resistance and to determine susceptibility and resistance breakpoint zone diameters for cefoxitin by using 51 methicillin susceptible Staphylococcus aureus (MSSA) and 63 methicillin resistant S. aureus (MRSA) isolates. In the study, while oxacillin and cefoxitin results were obtained within 4–7 h (5.5 h in average) for MSSA isolates, the results of MRSA isolates were obtained within 5.5–9 h (6.6 h in average) for both antibiotics on QC ES agar. QC ES agar is an inexpensive medium for rapid detection (4–9 h) of methicillin resistance by disc diffusion method using oxacillin or cefoxitin. Additional studies for further evaluation of the efficiency of QC-ES agar in rapid determination of methicillin resistance in S. aureus may be beneficial.

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Authors and Affiliations

  1. Medical School, Department of Medical Microbiology, Ondokuz Mayis University, 55139, Samsun, Turkey

    Ahmet Yilmaz Coban, Yeliz Tanrıverdı Caycı & Belma Durupınar

  2. Kyrenia Hospital, Clinical Microbiology Laboratory, Kyrenia, Cyprus

    Ugur Demırpek

  3. Faculty of Arts and Science, Department of Biology, Amasya University, Amasya, Turkey

    Tuba Yıldırım

  4. Medical School, Department of Medical Microbiology, Acibadem University, Maltepe, Istanbul, Turkey

    Tanil Kocagoz

Authors
  1. Ahmet Yilmaz Coban
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  2. Ugur Demırpek
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  3. Tuba Yıldırım
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  4. Yeliz Tanrıverdı Caycı
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  5. Tanil Kocagoz
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  6. Belma Durupınar
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Correspondence to Ahmet Yilmaz Coban.

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Coban, A.Y., Demırpek, U., Yıldırım, T. et al. Rapid detection of methicillin resistance in Staphylococcus aureus isolates; evaluation of colorimetric Quicolor ES agar and determination of breakpoint inhibition zone diameters of cefoxitin. World J Microbiol Biotechnol 27, 1901–1904 (2011). https://doi.org/10.1007/s11274-011-0649-y

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  • DOI: https://doi.org/10.1007/s11274-011-0649-y

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