Skip to main content
Log in

Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, a total of 80 rhizobacteria was isolated from coastal agricultural ecosystem of cultivated vegetable rhizosphere soils. The isolates were screened for antagonistic activity against Sclerotium rolfsii and Colletotrichum capsici and plant growth promoting traits. The results revealed that 15.0 and 43.7% isolates showed statistically significant inhibition of mycelial growth of S. rolfsii and C. capsici respectively, while 48.7% isolates produced siderophore, 57.5% isolates solubilized phosphate and 21.1% isolates produced indole-3-acetic acid more than 20 μg/mL. However, only three isolates PfS1, PfR2 and BL5 were found positive to all properties tested. The identification of potential bacterial isolates through Microbial Identification System (BIOLOG) and 16S rDNA sequencing of the isolates revealed Bacillus species were dominant in the cultivated vegetable rhizosphere soil of Neil and Havelock Islands, India.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Ahmed S (1995) Agriculture-fertilization interface in Asia issue of growth and sustainability. Oxford and IBH Publishing Co, New Delhi

    Google Scholar 

  • Chen WP, Kuo TT (1993) A simple and rapid method for the preparation of gram negative bacterial genomic DNA. Nucleic Acids Res 21:2260

    Article  CAS  Google Scholar 

  • Ding Y, Wang J, Liu Y, Chen S (2005) Isolation and identification of nitrogen fixing bacilli from plant rhizospheres in Beijing region. J Appl Microbiol 99:1271–1281

    Article  CAS  Google Scholar 

  • Edwards U, Rogall T, Blocker H, Emde M, Bottger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853

    Article  CAS  Google Scholar 

  • Garbeva P, van Veen JA, van Elsas JD (2003) Predominant Bacillus spp. in agricultural soil under different management regimes detected via PCR-DGGE. Microb Ecol 45:302–316

    Article  CAS  Google Scholar 

  • Glick BR (1995) The Enhancement of plant growth by free living bacteria. Can J Microbiol 41:109–117

    Article  CAS  Google Scholar 

  • Glick BR, Bashan Y (1997) Genetic manipulation of plant growth-promoting bacteria to enhance biocontrol of phytopathogens. Biotechnol Adv 15:353–378

    Article  CAS  Google Scholar 

  • Glick BR, Penrose DM, Li J (1998) A model for the lowering of plant ethylene concentrations by plant growth–promoting bacteria. J Theor Biol 190:63–68

    Article  CAS  Google Scholar 

  • Gordon SA, Paleg LG (1957) Quantitative measurement of indole acetic acid. Physiol Plant Pathol 10:347–348

    Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein 242 metabolism. Academic Press, New York, pp 21–132

    Google Scholar 

  • King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44:301–307

    CAS  Google Scholar 

  • Kloepper JW (1993) Plant growth-promoting rhizobacteria as biocontrol agents. In: Metting FB Jr (ed) Soil microbial ecology-applications in agricultural and environmental management. Marcel Dekker, Inc., New York, pp 255–274

    Google Scholar 

  • Laguerre G, Allard MR, Revoy F, Amarger N (1994) Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR amplifies 16S rRNA genes. Appl Environ Microbiol 60:56–63

    CAS  Google Scholar 

  • Nishijima T, Toyota K, Mochizuki M (2005) Predominant culturable Bacillus species in Japanese arable soils and their potential as biocontrol agents. Microb Environ 20:61–68

    Article  Google Scholar 

  • Patten CL, Glick BR (1996) Bacterial biosynthesis of Indole-3-Acetic Acid. Can J Microbiol 42:207–220

    Article  CAS  Google Scholar 

  • Principe A, Alvarez FA, Castro MG, Zachi L, Fischer SE, Mori GB, Jofre E (2007) Biocontrol and PGPR features in native strain isolated from saline soils of Argentina. Curr Microbiol 55:314–322

    Article  CAS  Google Scholar 

  • Rosch C, Mergel A, Bothe H (2002) Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil. Appl Environ Microbiol 68:3818–3829

    Article  CAS  Google Scholar 

  • Ross IL, Alami Y, Harvey PR, Achouak W, Ryder MH (2000) Genetic diversity and biological control activity of novel species closely related Pseudomonads isolated from wheat field soils in South Australia. Appl Environ Microbiol 66:1609–1616

    Article  CAS  Google Scholar 

  • Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Ann Biochem 160:47–56

    Article  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4888

    Article  CAS  Google Scholar 

  • Verma SC, Ladha JK, Tripathi AK (2001) Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. J Biotechnol 91:127–141

    Article  CAS  Google Scholar 

  • Wang YY, Brown HN, Crowley DE, Szaniszlo PJ (1993) Evidence for direct utilization of siderophore, ferioxamine b, in axenically grown cucumber. Plant Cell Environ 16:579–585

    Article  CAS  Google Scholar 

  • Xie GH, Cai MY, Guang CT, Steinberger Y (2003) Cultivable heterotrophic N2-fixing bacterial diversity in rice fields in the Yangtze River Plain. Biol Fertil Soils 37:29–38

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by grants from the project “Application of Microorganisms in Agriculture and Allied Sectors” of the NBAIM-ICAR, UP, Mau, India.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Krishna Kumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumar, K., Amaresan, N., Bhagat, S. et al. Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops. World J Microbiol Biotechnol 27, 1625–1632 (2011). https://doi.org/10.1007/s11274-010-0616-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-010-0616-z

Keywords

Navigation