Abstract
A total of 41 strains of lactic acid bacteria (LAB) isolated from durum wheat sourdoughs used to produce Cornetto di Matera bread, were identified by SDS-PAGE of whole cell proteins (WCP) and screened for acid production ability, antimicrobial activity and exopolysaccharide (EPS) production. The isolates were identified as Lactobacillus plantarum (49%), Leuconostoc mesenteroides (17%), Lactobacillus curvatus (15%), Lactobacillus paraplantarum (12%), Weissella cibaria (5%) and Lactobacillus pentosus (2%). Several strains of Lb. plantarum and Leuc. mesenteroides showed a high acid production ability. The antagonistic activity was tested using an agar-spot deferred antagonism assay against a set of five indicators. The species had different profiles of inhibition. Lb. plantarum had the largest spectrum of inhibition, while no isolates of W. cibaria and Leuc. mesenteroides showed antimicrobial activity. No strains had antimicrobial activity against Bacillus cereus. The inhibitory activity of five strains was confirmed to be sensitive to proteolytic enzymes and thus potentially due to bacteriocin production. All Leuc. mesenteroides and W. cibaria strains produced EPS from sucrose. Some Lb. plantarum and Lb. paraplantarum strains produced EPS from different sugars in solid media. EPS production in liquid media was different within the species, with the highest production in liquid media containing glucose and maltose. A defined strain starter culture (W. cibaria DBPZ1006, Lb. plantarum DBPZ1015 and S. cerevisiae MTG10) was selected on the basis of technological properties and tested in model sourdough fermentations.
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References
Arendt EK, Ryan LAM, Dal Bello F (2007) Impact of sourdough on the texture of bread. Food Microbiol 24:165–174
Bjorkroth KJ, Schillinger U, Geisen R, Weiss N, Hoste B, Holzapfel WH, Korkeala HJ, Vandamme P (2002) Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food clinical samples. Int J System Evol Microbiol 52:141–148
Catzeddu P, Mura E, Parente E, Sanna M, Farris GA (2006) Molecular characterization of lactic acid bacteria from sourdough breads produced in Sardinia (Italy) and multivariate statistical analyses of results. Syst Appl Microbiol 29:138–144
Corsetti A, Gobbetti M, Smacchi E (1996) Antibacterial activity of sourdough lactic acid bacteria: isolation of a bacteriocin-like inhibitory substance from Lactobacillus sanfrancisco C57. Food Microbiol 13:447–456
Corsetti A, Lavermicocca P, Morea M, Baruzzi F, Tosti N, Gobbetti M (2001) Phenothypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy. Int J Food Microbiol 64:95–104
Corsetti A, De Angelis M, Dellaglio F, Paparella A, Fox PF, Settani L, Gobbetti M (2003) Characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses. J Appl Microbiol 94:641–654
Corsetti A, Settanni L, Van Sinderen D (2004) Characterization of bacteriocin-like inhibitory substances (BLIS) from sourdough lactic acid bacteria and evaluation of their in vitro and in situ activity. J Appl Microbiol 96:521–534
De Vuyst L, Neysens P (2005) The sourdough microflora: biodiversity and metabolic interactions. Trends Food Sci Technol 16:1–14
De Vuyst L, Vancanneyt M (2007) Biodiversity and identification of sourdough lactic acid bacteria. Food Microbiol 24:120–127
De Vuyst L, Schrijvers V, Paramithiotis S, Hoste B, Vancanneyt M, Swings J, Kalantzopoulos G, Tsakalidou E, Messens W (2002) The biodiversity of lactic acid bacteria in Greek traditional wheat sourdoughs is reflected in both composition and metabolic formation. Appl Environ Microbiol 68:6059–6069
Di Cagno R, De Angelis M, Limitone A, Minervini F, Carnevali P, Corsetti A, Gänzle M, Ciati R, Gobbetti M (2006) Glucan and Fructan production by sourdough Weissella cibaria and Lactobacillus plantarum. J Agric Food Chem 54:9873–9881
Ferchichi M, Valcheva R, Prévost H, Onno B, Dousset X (2007) Molecular identification of the microbiota of French sourdough using temporal temperature gradient gel electrophoresis. Food Microbiol 24:678–686
Gobbetti M (1998) The sourdough microflora: interactions of lactic acid bacteria and yeasts. Trends Food Sci Technol 9:267–274
Gobbetti M, Corsetti A, Rossi J, La Rosa F, De Vincenzi S (1994) Identification and clustering of lactic acid bacteria and yeasts from wheat sourdoughs of Central Italy. Ital J Food Sci 1:85–94
Gobbetti M, Smacchi E, Fox P, Stepaniak L, Corsetti A (1996) The sourdough microflora. Cellular localization and characterization of proteolytic enzymes in lactic acid bacteria. Lebensm Wiss Technol 29:561–569
INSOR (2000) Atlante dei Prodotti tipici: il pane (Eds. Agra-Rai Eri) pag. 13. Roma, Italy
Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12:39–85
Kline L, Sugihara TF (1971) Microorganisms of the San Francisco sour dough bread process. II. Isolation and characterization of undescribed bacterial species responsible for the souring activity. Appl Microbiol 21:459–465
Korakli M, Rossmann A, Gänzle MG, Vogel RF (2001) Sucrose metabolism and exopolysaccharide production in wheat and rye sourdoughs by Lactobacillus sanfranciscensis. J Agric Food Chem 49:5194–5200
Korakli M, Pavlovic M, Gänzle MG, Vogel RF (2003) Exopolysaccharides and kestose production by Lactobacillus sanfranciscensis LTH2590. Appl Environ Microbiol 69:2073–2079
Lacaze G, Wick M, Cappelle S (2007) Emerging fermentation technologies: development of novel sourdoughs. Food Microbiol 24:155–160
Leenhardt F, Levrat-Verny M, Chanliaud E, Rémésy C (2005) Moderate decrease of pH by sourdough fermentation is sufficient to reduce phytate content of whole wheat flour through endogenous phytase activity. J Agric Food Chem 53:98–102
Messens W, De Vuyst L (2002) Inhibitory substances produced by Lactobacilli isolated from sourdoughs—a review. Int J Food Microbiol 72:31–43
Paramithiotis S, Gioulatos S, Tsakalidou E, Kalantzopoulos G (2007) Interactions between Saccharomyces cerevisiae and lactic acid bacteria in sourdough. Proc Biochem 41:2429–2433
Parente E, Grieco S, Crudele MA (2001) Phenotypic diversity of lactic acid bacteria isolated from fermented sausages produced in Basilicata (Southern Italy). J Appl Microbiol 90:943–952
Pepe O, Blaiotta G, Moschetti G, Greco T, Villani F (2003) Rope-producing strains of Bacillus spp. from wheat bread and strategy for their control by lactic acid bacteria. Appl Environ Microbiol 69:2321–2339
Pepe O, Blaiotta G, Anastasio M, Moschetti G, Ercolini D, Villani F (2004) Technological and molecular diversity of Lactobacillus plantarum strains isolated from naturally fermented sourdoughs. Syst Appl Microbiol 27:443–453
Piraino P, Ricciardi A, Lanorte MT, Malkhazova I, Parente E (2002) A procedure for data reduction in electrophoretic fingerprints. Biotechnol Lett 24:1477–1482
Piraino P, Ricciardi A, Salzano G, Zotta T, Parente E (2006) Use of unsupervised and supervised artificial neural networks for the identification of lactic acid bacteria on the basis of SDS-PAGE patterns of whole cell proteins. J Microbiol Methods 66:336–346
Randazzo CL, Heilig H, Restuccia C, Giudici P, Caggia C (2005) Bacterial population in traditional sourdough evaluated by molecular methods. J Appl Microbiol 99:251–258
Ricciardi A, Parente E, Clementi F (1997) A simple method for the screening of lactic acid bacteria for the production of exopolysaccharides. Biotechnol Tech 11:271–275
Ricciardi A, Parente E, Aquino M, Clementi F (1998) Use of desalting gel for the rapid separation of simple sugars from exopolysaccharides produced by lactic acid bacteria. Biotechnol Tech 12:649–652
Ricciardi A, Paraggio M, Salzano G, Andreotti G, De Fina M, Romano P (2002) La microflora di impasti utilizzati per la produzione del Cornetto di Matera e del Pane di Altamura. Tecnica Molitoria 8:780–787
Ricciardi A, Parente E, Piraino P, Paraggio M, Romano P (2005) Phenotypic characterization of lactic acid bacteria from sourdoughs for Altamura bread produced in Apulia (Southern Italy). Int J Food Microbiol 98:63–72
Rocha MJ, Malcata XF (1999) On the microbiological profile of traditional Portuguese sourdough. J Food Proteomics 62:1416–1429
Röcken W (1996) Applied aspects of sourdough fermentation. Adv Food Sci 18:212–216
Settanni L, Massitti O, Van Sinderen D, Corsetti A (2005) In situ activity of a bacteriocin-producing Lactococcus lactis strain. Influence on the interactions between lactic acid bacteria during sourdough fermentation. J Appl Microbiol 99:670–681
Tieking M, Gänzle M (2005) Exopolysaccharides from cereal-associated lactobacilli. Trends Food Sci Technol 16:79–84
Tieking M, Korakli M, Ehrmann MA, Gänzle MG, Vogel RF (2003) In situ production of exopolysaccharides during sourdough fermentation by cereal and intestinal isolates of lactic acid bacteria. Appl Environ Microbiol 69:945–952
Torodov S, Onno B, Sorokine O, Chobert JM, Ivanova I, Dousset X (1999) Detection and characterization of a novel antibacterial substance produced by Lactobacillus plantarum ST31 isolated from sourdough. Int J Food Microbiol 48:167–177
Vandamme P, Pot B, Gillis M, Kersters K, Swings J (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438
van Geel-Schutten GH., Flesch F, ten Brink B, Smith MR, Dijkhuizen L (1998) Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides. Appl Microbiol Biotech 50:697–703
Vogel RF, Knorr R, Muller MRA, Steudel U, Gänzle MG, Ehrmann MA (1999) Non-dairy lactic fermentations: the cereal world. Antonie van Leeuwenhoek 76:403–411
Zotta T, Piraino P, Ricciardi A, McSweeney PLH, Parente E (2006) Proteolysis in Model Sourdough Fermentations. J Agric Food Chem 54: 2567–2574
Zotta T, Ricciardi A, Parente E (2007) Enzymatic activities of lactic acid bacteria isolated from Cornetto di Matera sourdough. Int J Food Microbiol 115:165–172
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This research was supported by funds from Agenzia Lucana di Sviluppo e di Innovazione in Agricoltura (ALSIA), Italy
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Zotta, T., Piraino, P., Parente, E. et al. Characterization of lactic acid bacteria isolated from sourdoughs for Cornetto, a traditional bread produced in Basilicata (Southern Italy). World J Microbiol Biotechnol 24, 1785–1795 (2008). https://doi.org/10.1007/s11274-008-9671-0
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DOI: https://doi.org/10.1007/s11274-008-9671-0