Skip to main content
Log in

Phylogenetic group distribution among Escherichia coli isolated from rivers in São Paulo State, Brazil

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The phylogenetic group distribution of Escherichia coli strains isolated from the Sorocaba and Jaguari Rivers located in the State of São Paulo, Brazil, is described. E. coli strains from group D were found in both rivers while one strain from group B2 was isolated from the Sorocaba river. These two groups often include strains that can cause extraintestinal diseases. Most of the strains analyzed were allocated into the phylogenetic groups A and B1, supporting the hypothesis that strains from these phylogenetic groups are more abundant in tropical areas. Though both rivers are located in urbanized and industrialized areas where the main source of water pollution is considered to derive from domestic sewage, our results suggest that the major sources of contamination in the sampling sites of both rivers might have originated from animals and not humans.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Carson CA, Shear BL, Ellersieck MR, Schnell JD (2003) Comparison of ribotyping and repetitive extragenic palindromic-PCR for identification of fecal Escherichia coli from humans and animals. Appl Environ Microbiol 69(3):1836–1839

    Article  CAS  Google Scholar 

  • CETESB (2004) Relatório de qualidade de águas interiores do Estado de São Paulo––2003. CETESB, São Paulo http://www.cetesb.sp.gov.br

  • Clermont O, Bonacorsi S, Bingen E (2000) Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66(10):4555–4558

    Article  CAS  Google Scholar 

  • Dixit SM, Gordon DM, Wu XY, Chapman T, Kailasapathy K, Chin JJC (2004) Diversity analysis of commensal porcine Escherichia coli-associations between genotypes and habitat in the porcine gastrointestinal tract. Microbiology 150:1735–1740

    Article  CAS  Google Scholar 

  • Dobrindt U, Agerer F, Michaelis K, Janka A, Buchrieser C, Samuelson M, Svanborg C, Gottschalk G, Karch H, Hacker J (2003) Analysis of genome plasticity in pathogenic and commensal Escherichia coli isolates by use of DNA arrays. J Bacteriol 185:1831–1840

    Article  CAS  Google Scholar 

  • Dombek PE, Johnson LK, Zimmerley ST, Sadowsky MJ (2000) Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources. Appl Environ Microbiol 66:2572–2577

    Article  CAS  Google Scholar 

  • Escobar-Parámo P, Grenet K, Le Menac'h A, Rode L, Salgado E, Amorin C, Gouriou S, Picard B, Rahimy MC, Andremont A, Denamur E, Ruimy R (2004) Large-scale population structure of human commensal Escherichia coli isolates. Appl Environ Microbiol 70(9):5698–5700

    Article  Google Scholar 

  • Everitt BS (1977) The analysis of contingency tables. Chapman & Hall, London

    Google Scholar 

  • Fienberg SE (1978) The analysis of cross-classified categorical data. MIT Press, Massachussets

    Google Scholar 

  • Gauch HGJ (1982) Multivariate analysis in community ecology. Cambridge University Press, Cambridge

    Google Scholar 

  • Gordon DM, Cowling A (2003) The distribution and genetic structure of Escherichia coli in Australian vertebrates: host and geographic effects. Microbiology 149:3575–3586

    Article  CAS  Google Scholar 

  • Goullet PH, Picard B (1986) Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources. J Gen Microbiol 132:1843–1851

    CAS  Google Scholar 

  • Herzer PJ, Inouye S, Inouye M, Whittam TS (1990) Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. J Bacteriol 172:6175–6181

    CAS  Google Scholar 

  • Johnson JR, O'Bryan TT, Kuskowski MA, Maslow JN (2001) Ongoing horizontal and vertical transmission of virulence genes and papA alleles among Escherichia coli blood isolates from patients with diverse source bacteremia. Infect Immun 69:5363–5374

    Article  CAS  Google Scholar 

  • Johnson JR, Stell AL (2000) Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 181:261–272

    Article  CAS  Google Scholar 

  • Kaper JB, Nataro JP, Mobley HLT (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2:123–140

    Article  CAS  Google Scholar 

  • Müller EE, Ehlers MM, Grabow WOK (2001) The occurence of E. coli O157:H7 in South African water sources indended for direct and indirect human consuption. Water Res 35:3085–3088

    Article  Google Scholar 

  • Nowrouzian FL, Wold AE, Adlerberth I (2005) Escherichia coli strains belonging to phylogenetic group B2 have superior capacity to persist in the intestinal microflora of infants. J Infec Diseases 191:1078–1083

    Article  CAS  Google Scholar 

  • Ohno A, Marui A, Castro ES, Reyes AAB, Elio-Calvo D, Kasitani H, Ishii Y, Yamaguchi K (1997) Enteropathogenic bacteria in the La Paz River of Bolivia. Am J Trop Med Hyg 57:438–444

    CAS  Google Scholar 

  • Oksanen J, Kindt R, O'Hara RB (2005) Vegan: community ecology package version 1.6-9. http://cc.oulu.fi/~jarioksa/

  • Parry SM, Palmer SR (2000) The public health significance of VTEC O157. Symp Ser Soc Appl Microbiol 88:1S–9S

    Google Scholar 

  • Parveen S, Portier KM, Robinson K, Edmiston L, Tamplim MI (1999) Discriminant analysis of ribotype profiles of Escherichia coli for differentiating human and nonhuman sources of fecal pollution. Appl Environ Microbiol 65:3142–3147

    CAS  Google Scholar 

  • Picard B, Garcia JS, Gouriou S, Duriez P, Brahimi N, Bingen E, Elion J, Denamur E (1999) The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect Immun 67:546–553

    CAS  Google Scholar 

  • Pupo GM, Karaolis DKR, Lan R, Reeves PR (1997) Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies. Infect Immun 65(7):2685–2692

    CAS  Google Scholar 

  • R Development Core Team (2005) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org

  • Russo TA, Johnson JR (2003) Medical and economic impact of extraintestinal infections due to Escherichia coli: an overlooked epidemic. Microbes Infect 5:449–456

    Article  Google Scholar 

  • Selander RK, Korhonen TK, Vaisanen-Rhen V, Williams PH, Pattison PE, Caugant DA (1986) Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect Immun 52:213–222

    CAS  Google Scholar 

  • Simiyu KW, Gathura PB, Kyule MN, Kanja LW, Ombui JN (1998) Toxin production and antimicrobial resistance of Escherichia coli river water isolates. East Afr Med J 75:699–702

    CAS  Google Scholar 

  • Ter Braak CJF (1995) Ordination. In: Jongman RHG, Ter Braak CJF, van Torgeren OFR (eds) Data analysis in community and landscape ecology. Cambridge University Press, Cambridge, pp 91–173

    Google Scholar 

  • Turner SJ, Lewis GD, Bellamy AR (1997) A genomic polymorphysm located downstream of the gcvP gene of Escherichia coli that correlates with ecological niche. Mol Ecol 6:1019–1032

    Article  CAS  Google Scholar 

  • Venables WN, Ripley BD (2002) Modern applied statistics with S 4 edn. Springer, New York

    Google Scholar 

  • Vinten AJA, Lewis DR, McGechan M, Duncan A, Aitken M, Hill C, Crawford C (2004) Predicting the effect of livestock inputs of E. coli on microbiological compliance of bathing waters. Water Res 38:3215–3224

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grant 2000/05721-8 from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). R.H.O. had fellowship from CAPES. L.M.M.O. had research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Laura M. M. Ottoboni.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orsi, R.H., Stoppe, N.C., Sato, M.I.Z. et al. Phylogenetic group distribution among Escherichia coli isolated from rivers in São Paulo State, Brazil. World J Microbiol Biotechnol 24, 1573–1577 (2008). https://doi.org/10.1007/s11274-007-9644-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-007-9644-8

Keywords

Navigation