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Enhanced production of laccase in Coriolopsis rigida grown on barley bran in flask or expanded-bed bioreactor

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Abstract

In this work, the effect on laccase activity of adding xylidine, veratryl alcohol and copper sulphate to cultures of Coriolopsis rigida under submerged cultivation conditions have been investigated. The highest activities (approximately 6 × 105 nkat/l) were obtained when the inducers copper sulphate (2 mM) and xylidine (10 mM) were added simultaneously. In addition, operating in the optimal conditions, it was possible to maintain the sustained production of laccase (around 3 × 105 nkat/l) for successive repeated batch cultures in an expanded-bed laboratory scale bioreactor. On the other hand, in vitro phenol degradation by laccase obtained in the bioreactor was studied with/without an effective mediator 1-hydroxybenzotriazol (HBT). The presence of a radical mediator plays an important role inducing the degradation of phenol, and without mediator the polymerization of phenol was detected.

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Acknowledgements

This research was financed by the Spanish Ministry of Science and Technology and European FEDER (Project CTM2004–01539/TECNO).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Vigo, Lagoas Marcosende, 36310, Vigo, Spain

    Teresa Alcántara, Jose Gómez, Marta Pazos & M. Ángeles Sanromán

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  1. Teresa Alcántara
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  2. Jose Gómez
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  3. Marta Pazos
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  4. M. Ángeles Sanromán
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Correspondence to M. Ángeles Sanromán.

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Alcántara, T., Gómez, J., Pazos, M. et al. Enhanced production of laccase in Coriolopsis rigida grown on barley bran in flask or expanded-bed bioreactor. World J Microbiol Biotechnol 23, 1189–1194 (2007). https://doi.org/10.1007/s11274-006-9334-y

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