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Kinetic study of phenol hydroxylase and catechol 1,2-dioxygenase biosynthesis by Candida tropicalis cells grown on different phenolic substrates

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Abstract

When Candida tropicalis was grown on phenol, catechol or resorcinol, the highest levels of specific activity of phenol hydroxylase (EC. 1.14.13.7) and catechol 1,2-dioxygenase (EC. 1.13.11.1) were attained with phenol. With the three aromatic compounds tested, the yeast cells exhibited sharp peaks of specific activity of both enzymes at particular incubation times. Phenol-induced cells containing high levels of both enzymes were capable of degrading rapidly and without delay 4-chlorophenol and 2,6-dichlorophenol, and to a lesser extend pentachlorophenol. However, the yeast could not grow on chlorophenols as major carbon and energy source.

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Authors and Affiliations

  1. Departamento de Ingeniería Bioquímica, Col. Santa María la Rivera, Escuela Nacional de Ciencias Biológicas, del I.P.N., Carpio y Plan de Ayala, “Centro Operativo Naranjo”, Jaime Torres Bodet No. 142, D.F, México

    Deifilia Ahuatzi-chacón, Guadalupe Ordorica-morales, Nora Ruiz-ordaz, Eliseo Cristiani-urbina, Cleotilde Juárez-ramírez & Juvencio Galíndez-mayer

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  1. Deifilia Ahuatzi-chacón
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  2. Guadalupe Ordorica-morales
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  3. Nora Ruiz-ordaz
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  5. Cleotilde Juárez-ramírez
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Ahuatzi-chacón, D., Ordorica-morales, G., Ruiz-ordaz, N. et al. Kinetic study of phenol hydroxylase and catechol 1,2-dioxygenase biosynthesis by Candida tropicalis cells grown on different phenolic substrates. World Journal of Microbiology and Biotechnology 20, 695–702 (2004). https://doi.org/10.1007/s11274-004-2622-5

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