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Bioactivity of MWCNT in Conidia of Entomopathogenic Fungus Isaria fumosorosea

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Abstract

The bioactivity of three kinds of multi-walled carbon nanotubes (MWCNT) towards the conidia of entomopathogenic fungus Isaria fumosorosea was examined in an in vitro study. Commercial—raw and functionalized—carboxylated MWCNT were applied. A fungal conidia suspension was placed in contact with dispersed MWCNT over different time-periods. After contact with the nanomaterial, the conidia were cultured on dishes and both the linear vegetative mycelium growth and the sporulation and germination of the spores derived from the culture were investigated. Also, the pathogenicity of the conidia after contact with MWCNT was examined in relation to test larvae. No fungistatic activity of MWCNT relative to I. fumosorosea conidia was demonstrated. Conidia contact with MWCNT resulted in the following changes in vital processes in the subsequent culture compared to the control standard culture: (1) raw MWCNT limited mycelium inoculation, but the growth rate observed later in the log-phase was more intense; (2) after 24-h conidia contact with all MWCNT types, the mycelium sporulated the most intensively; longer contact resulted in sporulation process limitation. Germination of conidia after contact with the MWCNT was not significantly modified. Raw MWCNT potentiated conidia pathogenicity towards test insects. It was observed that carboxylation of MWCNT reduces the bioactivity of this nanomaterial towards the investigated conidia.

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Acknowledgments

We would like to thank Dr. Magdalena Oćwieja from the Institute of Catalysis and Surface Physicochemistry, PASci, and Dr. Olga Woźnicka from the Institute of Zoology, Jagiellonian University in Krakow, for their help in SEM and TEM imaging of the nanomaterials used in the study. The study was supported by University of Agriculture in Krakow Grant DS 3109/KOŚR.

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Authors and Affiliations

  1. Department of Agricultural Environment Protection, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120, Krakow, Poland

    Anna Gorczyca

  2. Department of Chemistry and Physics, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120, Krakow, Poland

    Marek J. Kasprowicz & Tadeusz Lemek

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  1. Anna Gorczyca
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  2. Marek J. Kasprowicz
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  3. Tadeusz Lemek
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Correspondence to Anna Gorczyca.

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Fig. A

TEM (left) and SEM (right) imaging of MWCNT used in the experiment (raw MWCNT – a, b; MWCNT(COOH)x A – c, d and MWCNT(COOH)x B – e, f) (GIF 724 kb)

High resolution image (TIFF 4791 kb)

Fig. B

AFM imaging of MWCNT(COOH)x A (left) and MWCNT(COOH)x B (right) used in the experiment (GIF 61 kb)

High resolution image (TIFF 6537 kb)

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Gorczyca, A., Kasprowicz, M.J. & Lemek, T. Bioactivity of MWCNT in Conidia of Entomopathogenic Fungus Isaria fumosorosea . Water Air Soil Pollut 226, 75 (2015). https://doi.org/10.1007/s11270-015-2350-5

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