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Transcriptional activation of Mink enteritis virus VP2 by the C-terminal of its NS1 protein

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Abstract

Mink enteritis virus (MEV) NS1 is a multidomain and multifunctional protein containing origin binding, helicase, and transactivation domains. In particular, parvoviral NS1 proteins are transactivators of the viral capsid protein promoter although the manner by which they exert these transactivation effects remained unclear. In this study, the region of the transactivation domain of the NS1 C-terminal was found located at aa 557 ~ 668 and any deletion within this region reduced the transactivation activity. A dominant negative mutation of the 63 aa deletion in the C-terminal of NS1 protein resulted in loss of ability to activate P38 and VP2-5′UTR in a dual-luciferase reporter assay system, a VP2 protein expression system, and within the whole MEV genome, independent of downstream genes. Additionally, a full-length MEV clone deficient in its NS1 C-terminal failed to rescue the virus, possibly due to the loss of integrity of DNA sequences interacting with NS1 protein, and expression of VP2 was also inhibited even when normal NS1 protein was supplied in trans.

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Funding

This work was supported by National Key Research and Development Program of China (2016YFD0501001).

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Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China

    Qianqian Xie, Jigui Wang, Ying Liu, Jun Su, Chenchen Gu, Jing Wu & Weiquan Liu

  2. Department of Geriatrics, the Eight Medical Centre, Chinese PLA General Hospital, Beijing, China

    Jun Xiao

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  1. Qianqian Xie
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  2. Jigui Wang
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  4. Jun Su
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  7. Jun Xiao
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Contributions

WL, JX, JW, and QX conceived and designed experiments. Material preparation, experiments, and data collection were performed by QX, YL, CG, and JW. The first draft of the manuscript was written by QX and revised JS, JX, and WL. All authors read and approved the final manuscript.

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Correspondence to Jun Xiao or Weiquan Liu.

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Xie, Q., Wang, J., Liu, Y. et al. Transcriptional activation of Mink enteritis virus VP2 by the C-terminal of its NS1 protein. Virus Genes 59, 100–108 (2023). https://doi.org/10.1007/s11262-022-01947-z

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  • DOI: https://doi.org/10.1007/s11262-022-01947-z

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