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Full-length genome characterization of a novel alphapartitivirus detected in the ectomycorrhizal fungus Hygrophorus penarioides

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Abstract

Virus populations of ectomycorrhizal fungi remain poorly studied. In the present study, we characterized a new partitivirus isolated from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides, named “Hygrophorus penarioides partitivirus 1” (HpPV1). The whole genome of HpPV1, determined by merging deep sequencing and RLM-RACE approaches, comprised two dsRNA segments of 2053 bp and 2072 bp, respectively. Both dsRNA genome segments included a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp), and a capsid protein (CP), respectively. Based on BLASTp search, the sequences of the RdRp and CP exhibits the highest similarity (67.49% and 75.61% identity, respectively) to those of partitiviruses identified from an ascomycetous ectomycorrhizal fungus Sarcosphaera coronaria. Phylogenetic analyses performed based on the CP and RdRp sequences demonstrated that HpPV1 clusters within a clade that includes members of the genus Alphapartitivirus, belonging to the family Partitiviridae. Here, we propose that HpPV1 is a new member of the genus Alphapartitivirus. This is the first study reporting on a new partitivirus identified from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides.

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Funding

This study was funded by the Ankara University, Coordinatorship of Scientific Research Projects with the project number 19B0430002.

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ES conceptualized, designed, and performed the experiments, analyzed the data, and wrote the manuscript. IA acquired the research grant for this study, collected and identified the fungus sample. ES and IA participated in revising the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ergin Sahin.

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The authors declare that they have no conflict of interest in this study.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Karel Petrzik.

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Sahin, E., Akata, I. Full-length genome characterization of a novel alphapartitivirus detected in the ectomycorrhizal fungus Hygrophorus penarioides. Virus Genes 57, 94–99 (2021). https://doi.org/10.1007/s11262-020-01814-9

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  • DOI: https://doi.org/10.1007/s11262-020-01814-9

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