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Transcriptional suppression of IE180 and TK promoters by the EP0 of pseudorabies virus strains Ea and Fa

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Abstract

In our transient expression assays, the IE180 and TK promoters were markedly suppressed by the EP0 of the pseudorabies virus strains Ea and Fa (EP0/Ea, EP0/Fa). This is in contrast with the transactivating activity of EP0 from strain YS-81 (EP0/YS-81) in previous studies. Amino acid sequence alignment revealed several mutations in both EP0/Ea and EP0/Fa compared with EP0/YS-81. Most remarkable is a two-amino acid substitution next to the RING finger domain which was considered to be important for the transactivating activity of EP0 in previous studies. To address the effect of the two-amino acid substitution on the function of EP0, reverse mutants of EP0/Ea and EP0/Fa were generated. The subsequent expression assays indicated that this substitution was at least in part responsible for the effect on the regulatory activity of EP0. Our data suggested that EP0 may regulate the expression of the same genes in different pseudorabies virus strains differently.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC, 30471293) and Hubei Natural Science Foundation (2007ABB015).

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Authors and Affiliations

  1. Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Hongshan District, Wuhan, 430070, China

    Hong Guo, Rui Zhou, Yang Xi, Shaobo Xiao & Huanchun Chen

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  1. Hong Guo
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  2. Rui Zhou
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  3. Yang Xi
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  5. Huanchun Chen
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Correspondence to Rui Zhou or Huanchun Chen.

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Guo, H., Zhou, R., Xi, Y. et al. Transcriptional suppression of IE180 and TK promoters by the EP0 of pseudorabies virus strains Ea and Fa. Virus Genes 38, 269–275 (2009). https://doi.org/10.1007/s11262-008-0320-3

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  • DOI: https://doi.org/10.1007/s11262-008-0320-3

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