Abstract
Ovarian tissue transplantation makes it possible to restore fertility; however, the success of this technique depends on the transplant region used. Therefore, this study aimed to evaluate the effect of two subcutaneous regions on canine ovarian transplantation, pinna (Pi) and neck (Ne), for 7 and 15 days. Ovaries collected by ovariosalpingohysterectomy were fragmented using a punch device. Fresh fragments were fixed, and the others were immediately grafted onto the animal itself in the Pi and Ne regions for 7 and 15 days. Recovered fragments were evaluated for histology (morphology, development and stromal density), picrosirius (collagen fibers), and immunohistochemistry (fibrosis and cell proliferation). The results showed that follicular normality rates were lower in Pi-7 (78%) vs. control (90%) and Pi-15 (86%), similar in Ne-7 (92%) and superior in Ne-15 (97%) compared to the control, with the effect of the region Ne (94%) superior (P < 0.05) to Pi (82%). Stromal density reduced in both regions vs. control but was similar within 15 days. Fragments from both regions showed higher fibronectin labeling and deposition of type I and lower type III collagen fibers (P < 0.05) vs. control. Proliferation rates in Ne-7 were higher (P < 0.05) than in control, and Pi-15 was higher (P < 0.05) than Ne-15. In conclusion, the pinna may be a region with greater potential than the neck after a 15-day autotransplantation of canine ovarian tissue.
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Not applicable. All data supporting the findings of this study are included within the manuscript.
Abbreviations
- HVSBC-UECE:
-
Veterinary Hospital Prof. Sylvio Barbosa Cardoso of the State University of Ceará
- MEM-HEPES:
-
Minimum Essential Medium-buffers
- OSH:
-
Ovariosalpingohysterectomy
- PAF:
-
Paraformaldehyde
- PCNA:
-
Proliferating cell nuclear antigen
- TVT:
-
Transmissible venereal tumor
- SEM:
-
Standard error of the mean
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The authors are grateful for the support of the State University of Ceará (UECE), and collaborators Zoonosis Surveillance Unit (UVZ-Fortaleza/Brazil), Veterinary Hospital Prof. Sylvio Barbosa Cardoso of the State University of Ceará (HVSBC-UECE), Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA).
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The main experimental conception and design were created by Kele Amaral Alves. All laboratory procedures and surgeries were carried out by [Fabiana Aparecida Santilli Brandão], [Danielle Cristina Calado de Brito], [Leda Maria Costa Pereira], [Lucy Vanessa Sulca Ñaupas], [Samara Silva de Sousa], [Duanny Murinelly de Souza Cunha], and [Reginaldo Pereira de Sousa Filho]. Contributed reagents/materials: [Ana Paula Ribeiro Rodrigues] and [Dárcio Ítalo Alves Teixeira]. Data collection, statistical analyses, and interpretation were performed by [Fabiana Aparecida Santilli Brandão], [Benner Geraldo Alves], [Kele Amaral Alves], and [Danielle Cristina Calado de Brito]. Manuscript writing and proofreading: [Fabiana Aparecida Santilli Brandão], [Danielle Cristina Calado de Brito], [Lucy Vanessa Sulca Ñaupas], [Benner Geraldo Alves], and [Kele Amaral Alves]. All authors have read and approved the final manuscript.
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All animals were obtained from the Zoonoses Surveillance Unit of Fortaleza, in the State of Ceará, Brazil, with approval and realization under the guidelines of the Ethics Committee for the Use of Animals of the State University of Ceará (3349245/2018).
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Brandão, F.A., de Brito, D.C., Pereira, L.M. et al. Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue. Vet Res Commun 47, 1893–1905 (2023). https://doi.org/10.1007/s11259-023-10139-5
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DOI: https://doi.org/10.1007/s11259-023-10139-5